| 微波萃取——分光光度法测定萹蓄中的总黄酮 |
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| 引用本文： | 张丽敏,张金生,李丽华,李艳南.微波萃取——分光光度法测定萹蓄中的总黄酮[J].辽宁石油化工大学学报,2011(3). |
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| 作者姓名： | 张丽敏 张金生 李丽华 李艳南 |
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| 作者单位： | 辽宁石油化工大学化学与材料科学学院;辽宁石油化工大学石油化工学院; |
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| 摘 要： | 采用微波辅助萃取法萃取萹蓄中的总黄酮,以芦丁为对照品,用分光光度法测定总黄酮的含量。利用正交实验考察了萃取温度、料液比、乙醇体积分数和回流时间对总黄酮含量的影响。实验结果表明:最佳条件为萃取温度80℃、料液比1∶40、乙醇体积分数为85%、回流时间7min。在最优条件下测得总黄酮的平均质量分数为2.099mg/g,总黄酮的平均萃取率为3.481%,相对标准偏差为0.304%(n=6),回收率为94.17%～99.50%。
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| 关 键 词： | 微波萃取 萹蓄 总黄酮 分光光度法 正交 |
Determination of Flavonoids From Polygo-Num Avculare by Microwave-Assisted Extraction and Spectrophotometry |
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| ZHANG Li-min,ZHANG Jin-sheng,LI Li-hua,LI Yan-nan.Determination of Flavonoids From Polygo-Num Avculare by Microwave-Assisted Extraction and Spectrophotometry[J].Journal of Liaoning University of Petroleum & Chemical Technology,2011(3). |
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| Authors: | ZHANG Li-min ZHANG Jin-sheng LI Li-hua LI Yan-nan |
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| Affiliation: | ZHANG Li-min1,ZHANG Jin-sheng2,LI Li-hua2,LI Yan-nan1(1.School of Chemistry and Materials Science,Liaoning Shihua University,Fushun Liaoning 113001,P.R.China,2.School of Petrochemical Technology,P.R.China) |
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| Abstract: | Flavonoids were extracted from polygonum avculare by microwave-assisted extraction,and its content was determined by spectrophotometry using the rutin as the quality control standard.The effect of extraction temperature,solid to liquid ratio,alcohol concentration and extraction time on content determination were investigated by orthogonal.The optimum conditions are as follows: extraction temperature 80 ℃,the solid to liquid 1∶40,the volume ratio of ethanol 85% and extraction time 7 min.Under the optimum conditions,the average content of flavonoids in polygonum avculare is 2.099 mg/g,average total flavonoids extract ratio is 3.481%,RSD= 0.304%(n=6),and their recovery rate measured are in the range of 94.17% ~99.50%. |
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| Keywords: | Microwave-assisted extraction Polygonum avculare Flavonoids Spectrophotometry Orthogonal |
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