| 剖析分光光度法测定大米中直链淀粉含量 |
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| 引用本文： | 尹凤芝. 剖析分光光度法测定大米中直链淀粉含量[J]. 生命科学仪器, 2012, 10(1): 10-13 |
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| 作者姓名： | 尹凤芝 |
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| 作者单位： | 农业部乳品质量监督检验测试中心哈尔滨,黑龙江哈尔滨,150078 |
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| 摘 要： | 将大米粉碎后，再对粉碎试样脱脂，脱脂后的试样加入氢氧化钠溶液，然后再加入碘试剂，用分光光度法在720nto处测定吸光度。利用马铃薯直链淀粉和支链淀粉的混合标样制作校正曲线，从校正曲线中可知样品中直链淀粉含量。
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| 关 键 词： | 直链淀粉 支链淀粉 碘试剂 分光光度法 校正曲线 |
Analysis on the determination of amylose content in rice by spectrophotometry |
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| YIN feng zhi. Analysis on the determination of amylose content in rice by spectrophotometry[J]. Life Science Instruments, 2012, 10(1): 10-13 |
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| Authors: | YIN feng zhi |
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| Affiliation: | YIN feng zhi (Milk Determination and Test Center of Agricultural Development of I-Iei long jiang province ,,Harbin 150078) |
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| Abstract: | To crumble to dust of the rice, To smash the specimen to skim, adding NaOH solutions to the skim the sample, then add the Iodine reagents, spectrophotometry determination of the absorbance at 720 nm o Make use of amylose and amglooectin to do calibration curve, from calibration curve it can be know amylose content of the sample o |
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| Keywords: | amylose amylopectin Iodine reagents spectrophotometry calibration curve |
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