Increased Lipid Peroxidation in LDL from Type-2 Diabetic Patients |
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Authors: | Romain Colas Myriam Moret " target="_blank">Hubert Vidal " target="_blank">Philippe Moulin " target="_blank">Michel Lagarde " target="_blank">Catherine Calzada |
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Affiliation: | 1.Université de Lyon,Lyon,France;2.INSA-Lyon, RMND,Villeurbanne,France;3.INSERM U870, INSA-Lyon, Régulations métaboliques, Nutrition et Diabètes,Bat Louis Pasteur, INSA-Lyon, IMBL,Villeurbanne,France;4.INRA U1235,Oullins,France;5.Hospices Civils de Lyon,Lyon,France |
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Abstract: | Increased oxidative stress is associated with type-2 diabetes and related cardiovascular diseases, but oxidative modification
of LDL has been partially characterized. Our aim was to compare the lipid and fatty acid composition as well as the redox
status of LDL from diabetic patients and healthy subjects. First, to ensure that isolation of LDL by sequential ultracentrifugation
did not result in lipid modifications, lipid composition and peroxide content were determined in LDL isolated either by ultracentrifugation
or fast-protein liquid chromatography. Both methods resulted in similar concentrations of lipids, fatty acids, hydroxy-octadecadienoic
acid (HODE) and malondialdehyde (MDA). Then, LDLs were isolated by ultracentrifugation from eight type-2 diabetic patients
and eight control subjects. Compared to control LDL, diabetic LDL contained decreased cholesteryl esters and increased triglyceride
concentrations. Ethanolamine plasmalogens decreased by 49%. Proportions of linoleic acid decreased in all lipid classes, while
proportions of arachidonic acid increased in cholesteryl esters. Total HODE concentrations increased by 56%, 12- and 15-hydroxy-eicosatetraenoic
acid by 161 and 86%, respectively, and MDA levels increased by twofold. α-Tocopherol concentrations, expressed relative to
triglycerides, were lower in LDL from patients compared to controls, while γ-tocopherol did not differ. Overall, LDL from
type-2 diabetic patients displayed increased oxidative stress. Determination of hydroxylated fatty acids and ethanolamine
plasmalogen depletion could be especially relevant in diabetes. |
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