地高辛抗血清对缺氧损伤心肌组织内源性洋地黄样因子和心肌细胞膜ATP酶活性的影响

目的 评价地高辛抗血清对心肌缺氧损伤的保护作用与机制 。方法 制备心肌组织缺氧损伤模型,观察不同剂量的地高辛抗血清对缺氧损伤心肌组织内源性洋地黄样因子水平和心肌细胞膜ATP酶活性的影响。结果 缺氧损伤可使心肌组织内源性洋地黄样因子水平明显升高,心肌细胞膜ATP酶活性明显下降;地高辛抗血清能明显拮抗缺氧对心肌细胞膜 ATP酶活性的抑制作用,使酶活性得到恢复 。结论 缺氧损伤所致心肌组织内源性洋地黄样因子水平升高是缺氧介导心肌损伤的分子生物学基础,地高辛抗血清通过拮抗内源性洋地黄样因子的作用,减轻缺氧所致心肌损伤,对缺氧损伤心肌具有保护作用 。

Effect of anti-digoxin antiserum on endogenous digitalis-like factor and membrane ATPase activity in hypoxic injury myocardium1

Aim To evaluate the protective effect of anti-digoxin antiserum on hypoxic injury my-ocardium and its mechanism.Methods It was observed that different concentration an-ti-digoxin antiserum effect on endogenous digitalis-like factor and cell membrane ATPase ac-tivity in hypoxic myocardium model.Results The level of endogenous digitalis-like factor was remarkably higher,cell membrane ATPase activity were remarkably lower in hypoxic group than those of normal group;anti-digoxin antiserum can resume membrane ATPase ac-tivity.Conclusion Rise of endogenous digitalis-like factor was basic of molecular biology of myocardial damage during myocardial hypoxia.Anti-digoxin antiserum has lightened my-ocardial injury and has protective effect on hypoxic myocardium by against effect of endoge-nous digitalis-like factor.