降纤酶对大鼠脑缺血再灌注损伤保护作用的实验研究

目的 探讨降纤酶对脑缺血再灌注损伤的保护作用及其作用机制。方法 肾血管型高血压大鼠(RHR)70只,随机分为降纤酶组、对照组和伪手术组,按Longa氏方法改良复制成可再灌注的大脑中动脉闭塞(MCAO)模型,缺血2h 后恢复灌注。降纤酶组经股静脉给予降纤酶10U·kg^-1体重,对照组给予等量的生理盐水。脑片行TTC及HE染色,观察两组在不同时间点的梗死灶的大小、脑微血管损害的表现及并发出血情况。结果 降纤酶组的梗死灶的体积较对照组明显缩小,脑微血管损害减轻,镜下出血灶减少。结论 降纤酶具有保护脑缺血再灌注损伤的作用。

Experimental studies on the protective effect of defibrase against reperfusion injury after cerebral ischemia

Aim To ascertain whether defibrase has the protective effect against reperfusion injury after cerebral i schemia.Methods 70 renovascular hypertensive rats(RHR) were randomly divided into defibrase group, control grouPand sham-operated group.Reversible middle cerebral artery occlusion(MCAO)models were produced by the modified.Long as method ,and reperfusion was begun 2 hours after occlusion.Rats in the defibrase grouPwere given defibrase 10 U·kg^-1body weight via femonal intraveneous injection, and in the control grouPwith the same amount of saline.The brain pieces were processed by TTC and HE staining and the infarct size, brain micro vessels damage and secondary bleeding were compared between the two groups.Results The volume of infarction in the defibrase grouPwas obviously smaller than in the control group, the damage of brain micro vessels was less severe ,and the bleeding lesions under optical microscope were less than in the control group.Conclusion Defibrase has protective effect against reperfusion injury post cerebral ischemia.