SD大鼠血管内皮细胞损伤模型的制作方法1

目的 建立SD大鼠血管内皮细胞损伤模型。方法 SD大鼠随机分为正常对照组和模型组。模型组大鼠皮下注射稀释1.5倍的肾上腺素0.05 mg^-1·100g^-1(tid),连续5d。于d4起,每2次给药间将大鼠置于0°C冰水中5 min。正常对照组则皮下注射等量NS。于d6,2组大鼠自颈总动脉取血,分别测定CEC计数、t-PA、PAI活性、6-keto-PGF.含量及血小板最大聚集率。结果 模型组较正常对照组大鼠CEC计数、PAI活性、血小板最大聚集率明显升高(P<0.01),t-PA活性、6-keto-PGFn.含量降低(P<0.01;P<0.05)。结论 大 剂量肾.上腺素加冰泳刺激能造成SD大鼠血管内皮细胞损伤。

Establishment of a model of the vascular endothelial cell injury in SD rats

Aim To establish a model of the vascular endothelial cell (VEC) injury in SD rats. Methods SD rats were randomly divided into the control and the model groups. The model rats were injected with adrenaline diluted to 2.5 times0.05 mg·100 g^-1(tid) for 5 d continously. From the 4th d, they were irritated for 5 min in the 0°C cold-water in the middle between adrenaline injections. The control rats were given 0.9% NS as above. At 6th d, blood samples were taken from carotid arteries of the rats and the CEC counts, t-PA、PAI activities, 6 keto-PGF1 concentrations and the platelet aggregation rate_(max) were detected respectively.Results In the model group, as compared with those in the control group, t-PA activity and 6-keto-PGF1. concentration decreased significantly(P<0.01,P<0.05),while the CEC count、PAI activity and the platelet aggregation rate_(max) increased obviously (P<0.01).Conclusions A large dose of adrenaline in combination with cold-water itritation can cause the VEC injury in SD rats.