DDPH对多种介质所致豚鼠离体肠道平滑肌收缩的影响

目的 研究1-(2, 6-二甲基苯氧基)-2-(3, 4-二甲氧基苯乙氨基)丙烷盐酸盐(DDPH)对多种介质引起的豚鼠离体肠道平滑肌收缩的影响。方法 肠道平滑肌实验法。结果 不同剂量的DDPH 可非竞争性抑制磷酸组胺、氯化乙酰胆碱及Ca²⁺的作用, 使量效曲线非平行右移, 最大反应压低, 其pD₂'值分别为4.0、3.8、3.9。DDPH 还可抑制K⁺所致肠道平滑肌收缩, 但作用较维拉帕米弱, IC₅₀ 值分别为2.7μmol·L^-1 和0.7 μmol·L^-1。结论 DDPH通过非竞争性Ca²⁺拮抗作用使豚鼠回肠平滑肌松弛。

Inhibitory effects of DDPH on contraction of isolated ileum of guinea pigs induced by some mediators

Aim To investigate the effects of 1-(2, 6-dimethy lphenoxy)-2-(3, 4-dime thox ypheny-lethy lamino)pro pane hydrochloride (DDPH)on cont raction of isolated ileum of guinea pigs induced by some mediators.Methods The routine methods for experiment on contraction-effect of the isolated ileum were emplayed.Results Histamine, acetyl choline and CaCl₂ separetely caused a significant cumulative concentration-related effect on ileum constraction with the pD₂ values being 7.7、9.8 and 5.3 respectively.after being incubated with DDPH of different dosage for 15 min, the responses elicited by Hist.ACh and CaCl₂ were depressed and the pD₂'values were 4.0, 3.8 and 3.9 respectively.Cumulative concent ration of DDPH and verapamil Resulted in a concentration dependent relaxing effect on contraction of ileum induced by K⁺ 60 mmol·L^-1 with the IC₅₀ values being 2.7 μmol·L^-1 and 0.7 μmol·L^-1. Conclusion DDPH has significant relaxing effect and a calcium antagonistic effect on ileum.