总丹酚酸对小鼠脑缺氧的保护作用1

目的 观察总丹酚酸(total salvianolic acids,Salt) 对小鼠脑缺或的保护作用。方法 利用小鼠断头及亚峭酸钠造成急性脑缺氧模型, 观察Sal 的杭脑缺或作用, 并以连二亚硫酸钠造成培养神经细胞缺氧损伤模型, 初步探讨.Sal的作用机制。结果 Sallo,20 m g·k g^-1iv 能明显延长小鼠断头张口喘气时间及亚峭酸钠致缺筑小鼠的生存时间, 并显著降低急性脑缺氧小鼠脑组织的丙二醛(MDA) 含量。Sal 1~10μg·L^-1·能剂量依赖地降低神经细胞缺氧性损伤时的细胞死亡率, 减少乳酸脱氛酶(LDH) 的释放, 并能明显减少MDA 的生成。结论 Sal能明显对杭小鼠急性脑缺氧, 其机理可能与其减少脂质过氧化物生成有关。

Protective effects of total salvianolic acids on cerebral hypoxia in mice

Aim The protective effect of total salvianolic acids (Sal) on cerebral hypoxia in mice was studied. Methods Acute cerebral hypoxia was induced by sodium nitrite sc and decapitation. The effect of Sal on acute cerebral hypoxia in mice and neuronal hypoxia injury induced by sodium dithionite in primary cultures were observed.Results Sal in the doses of 10, 20 mg·kg^-1 iv protected mice against the acute cerebral hypoxia and inhibited the production of lipid peroxidation in brain tissue of mice caused by cerebral hypoxia. Sal in the doses of 1~ 10μg·L^-1 reduced the rate of cell death and the content of MDA and lowered LDH content in extra-cellular bathing media in oxygen deprived cortical cultures.Conclusions Sal protects mice against cerebral hypoxia by suppressing the generation of lipid peroxide.