胎盘提取液影响脂蛋白-胆固醇代谢的实验研究

目的 探讨人胎盘提取液对高脂血症大鼠的脂蛋白-胆固醇代谢、过氧化作用及血小板聚集功能的影响及其作用机制。方法 建立Wistar大鼠高血脂模型,用胎盘提取液0.4ml·(100g·d)^-1连续灌胃12d,测定其血清TG、TC、LDL-C、HDL-C及其亚组分HDL2-C的含量;测定全血及肝组织LPO、SOD的活性,并通过脂肪染色观察提取液对肝脏脂肪沉积的影响;通过放免法测定血浆中6-酮-PGF1α、TXB2含量及血小板最大聚集率。结果 胎盘提取液使高脂鼠HDL-C、HDL2-C含量明显升高(P<0.01);TG、TC、LDL-C含量明显降低(P<0.01);明显降低高脂鼠血清及肝组织LPO含量(P<0.01),提高SOD活性(P<0.01);抑制肝脏脂肪沉积,使脂肪空泡明显减少;使血浆中PGI2含量明显升高(P<0.01),TXB2含量无明显变化,并明显降低血小板聚集率(P<0.01)。结论 胎盘提取液明显改善高脂大鼠脂蛋白-胆固醇代谢,增强机体抗氧化能力,抑制血小板聚集,并抑制脂肪肝形成,有利于拮抗动脉粥样硬化的发生和发展。

Effect of human placental extract on the lipoprotein-cholesterol metabol ism

Aim The Effect and mechanismof human placental extract(HPE) on the lipoprotein-c holesterol metabolism, peroxidation and the function of platelet agg reg ation in hyperlipaemiarats wereabserved.Methods Wistarrat with hyperlipaemia models weregiven each HPE 0.4 ml (100 g)^-1·d^-1 throug h lavage for 12 days.The serum levels of TG, TC, LDL-C, HDL-Cand HDL 2-C in its subgroup weremeasured.The activies of LPO and SOD in both blood and liver tissue weredeter mined.The Effect of HPE on lipidosis of liver wereabserved by fat dy eing.The levels of 6-keto-PG F1α, TXB 2 in plasma and maximum platelet agg regation rate were measured by ELASA.Result The levels of HDL-Cand HDL2-C wereincreased (P<0.01)whilethe levels of TG, TCand LDL-C weredecreased (P<0.01).The levels of LPO in serum and liver tissue decreased obviously and SOD activity increased (P<0.01).The lipidosis in liver was inhibited and adipose hollow spacereduced obviously.The levels of PG I2 in plasma increased (P<0.01), the level of T XB 2 was no t changed and platelet agg reg ation rate was decreased obviously.Conclusion HPE may improve the lipoprotein-cholesterol metabolism, enhance the antioxidation andinhibite platelet aggregation and the formation of lipidosis of liver in hyperlipemiarats.HPE is good for inhibition of the formation and development of atheoscleorsis.