| 磺胺母核人工抗原的制备 |
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| 引用本文： | 高梓翔,马鑫添,李秀丽.磺胺母核人工抗原的制备[J].广东化工,2012,39(1):7-7,34. |
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| 作者姓名： | 高梓翔 马鑫添 李秀丽 |
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| 作者单位： | 华南理工大学化学化工学院,广东广州,510640 |
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| 摘 要： | 吡啶为溶剂,用对氨基苯环酰胺和丁二酸酐为原料合成磺胺母核的半抗原（H）,产物结构通过HNMR确证。再用牛血清白蛋白（BSA）通过碳二亚胺法合成具有诱导产生抗体能力的免疫原（H-BSA）,通过紫外光谱定性测定以及间接ELISA检测H-BSA证明成功与否。磺胺母核的半抗原合成的最佳实验条件是：温度60℃,溶剂选用吡啶,反应时间6 h,加热方式为油浴加热。以期用H-BSA免疫家兔,制备多克隆抗体,为胶体金研究奠定基础。
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| 关 键 词： | 对氨基苯环酰胺丁二酸酐 HNMR 牛血清白蛋白 半抗原 免疫原 |
Preparation of Artificial Antigens for Sulfanilamides |
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| Gao Zixiang,Ma Xintian,Li Xiuli.Preparation of Artificial Antigens for Sulfanilamides[J].Guangdong Chemical Industry,2012,39(1):7-7,34. |
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| Authors: | Gao Zixiang Ma Xintian Li Xiuli |
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| Affiliation: | (South China University of Technology,School of Chemistry and Chemical Engineering,Guangzhou 510640,China) |
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| Abstract: | The hapten of sulfonamides（H） was synthesized by using sulfanilamide and succinic anhydride where pyridine is used as dissolvent,which is proved by HNMR.Moreover,H-BSA was synthesized by catodiimide method with Bovine Serum Albumin（BSA） and H.And the H-BSA can be proved by UV scan and indirect ELISA method that whether it was coupling success.The optimal experiment condition for preparation of H is using pyridine as dissolvent and heated with oil bath at temperature 60 ℃ for six hours.With the hope that we could get polyclonal antibody by immunizing rabbits with H-BSA.It will lay the foundations for the research of colloidal gold. |
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| Keywords: | sulfanilamide succinic anhydride BSA HNMR hapten immunogen |
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