| 长双歧杆菌BBMN68寡糖利用预测与验证 |
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| 引用本文： | 欧扬雯珊,刘松玲,刘治麟,陈巧燕,夏兵兵,赵亮,.长双歧杆菌BBMN68寡糖利用预测与验证[J].中国食品学报,2020,20(1):40-51. |
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| 作者姓名： | 欧扬雯珊 刘松玲 刘治麟 陈巧燕 夏兵兵 赵亮 |
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| 作者单位： | 教育部功能乳品重点实验室中国农业大学食品科学与营养工程学院;北京市高等学校畜产品工程技术研究中心;北京和益源生物技术有限公司;深圳市晨光乳业有限公司; |
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| 基金项目： | 北京市自然科学基金项目（6182024） |
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| 摘 要： | 长双歧杆菌BBMN68是来源于中国广西巴马长寿老人肠道的益生菌。本研究利用生物信息学技术分析该菌株寡糖糖苷水解酶基因,并利用生长试验及RT-PCR方法验证菌株对寡糖的利用。结果显示,该菌株具有多种糖苷水解酶基因,具有分解含半乳糖、葡萄糖、N-乙酰葡萄糖胺、N-乙酰半乳糖胺、果糖、甘露糖、阿拉伯糖和木糖单体的寡糖的潜质。生长试验表明,菌株可在低聚木糖、低聚半乳糖、低聚异麦芽糖、低聚麦芽糖、低聚果糖中良好生长。RT-PCR试验结果表明,不同条件下,寡糖转运系统基因BBMN68_74,BBMN68_75,BBMN68_217与BBMN68_925等16个基因可被不同程度地诱导表达。
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| 关 键 词： | BBMN68 寡糖 糖苷水解酶 碳水化合物转运子 |
The Prediction and Verification of Oligosaccharides on Bifidobacterium longum subsp. longum BBMN68 |
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| Ou Yangwenshan,Liu Songling,Liu Zhilin,Chen Qiaoyan,Xia Bingbing,Zhao Liang.The Prediction and Verification of Oligosaccharides on Bifidobacterium longum subsp. longum BBMN68[J].Journal of Chinese Institute of Food Science and Technology,2020,20(1):40-51. |
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| Authors: | Ou Yangwenshan Liu Songling Liu Zhilin Chen Qiaoyan Xia Bingbing Zhao Liang |
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| Affiliation: | (Key Laboratory of Functional Dairy,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083;Beijing Higher Institution Engineering Research Center of Animal Product,Beijing 100083;Beijing Heyiyuan Biotechnology Co.,Ltd,Beijing 100088;Shenzhen Chenguang Dairy Co.,Ltd,Shenzhen 518107,Guangdong) |
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| Abstract: | Bifidobacterium longum subsp.longum BBMN68 was a probiotic separated from intestine in centenarians in Bama,Guangxi,China.The article used bioinformatics technique to analyze the oligosaccharide glycose hydrolase genes,and validated the usage of relevant oligosaccharides via growth experiment and RT-PCR.Results showed that BBMN68 had various glycoside hydrolases,which may potentially degrade oligosaccharides into monosaccharides including galactose,glucose,N-acetylglucosamine,N-acetylgalactosamine,fructose,mannose,arabinose and xylose.BBMN68 can grow in a variety of oligosaccharide mediums including XOS,GOS,IMO,D-maltose and FOS.Oligosaccharide transporter genes(BBMN68_74,BBMN68_75,BBMN68_217,BBMN68_925,etc.)were induced to express via RT-PCR in different experimental conditions. |
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| Keywords: | BBMN68 oligosaccharide glucoside hydrolase carbohydrate transporter |
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