| 靶向蛋白质组学质谱技术在食物过敏原定量检测中的应用 |
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| 引用本文： | 蒋易蓉,任一平,陆柏益,. 靶向蛋白质组学质谱技术在食物过敏原定量检测中的应用[J]. 中国食品学报, 2020, 20(1): 302-310 |
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| 作者姓名： | 蒋易蓉 任一平 陆柏益 |
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| 作者单位： | 山西农业大学食品科学与工程学院;山西白酒生物工程研究生教育创新中心;山西杏花村汾酒厂股份有限公司; |
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| 基金项目： | 国家重点研发计划项目（2016YFD0400503）；山西省应用基础研究计划项目（201901D211377）；山西省高等学校科技成果转化培育项目；吕梁市引进高层次科技人才专业科技创新平台建设项目（2018-21-04）；山西省重点研发计划重点项目（201703D211001-06-03） |
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| 摘 要： | 食物过敏原的定量检测是实现食物过敏风险评估与风险管理的重要技术保障。现有国家标准中食物过敏原的检测方法主要为聚合酶链式反应(PCR)法和酶联免疫吸附(ELISA)法。针对现有方法存在的不足与缺陷,本文着重介绍了靶向蛋白质组学质谱技术及其在食物过敏原定量检测中的应用,分析该技术的优缺点及应用前景,为食物过敏原定量检测方法的建立提供依据。
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| 关 键 词： | 食物过敏原 靶向蛋白质组学 同位素稀释 质谱技术 定量检测 |
Application of Targeted Proteomics in Accurate Quantitation of Food Allergens |
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| Jiang Yirong,Ren Yiping,Lu Baiyi. Application of Targeted Proteomics in Accurate Quantitation of Food Allergens[J]. Journal of Chinese Institute of Food Science and Technology, 2020, 20(1): 302-310 |
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| Authors: | Jiang Yirong Ren Yiping Lu Baiyi |
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| Affiliation: | (College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058;Yangtze Delta Region Institute of Tsinghua University,Jiaxing 314000,Zhejiang) |
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| Abstract: | Accurate quantitation of food allergens is the crucial technical guarantee for risk assessment and risk management of food allergy.Food allergens detection method in present national standards mainly based on polymerase chain reaction(PCR)and enzyme-linked immunosorbent assay(ELISA)method.Focusing on the shortcomings of the present methods,this paper emphatically introduces the mass spectrometry-based targeted proteomics and its application in the food allergen quantitation,analyzes the advantages,disadvantages and the application prospects of this technology in food allergens quantitation,facilitating the research and establishment of food allergens quantitation methods. |
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| Keywords: | food allergens targeted proteomics isotope dilution mass spectrometry accurate quantitation |
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