Acquisition of amino acids by Lactobacillus delbrueckii subsp. bulgaricus 2038 when grown in the presence of casein |
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| Affiliation: | 1. Laboratory of Medical Foods, Shanghai Institute of Planned Parenthood Research, 2140 Xie-Tu Road, Shanghai 200032, China;2. Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai Zhang Jiang Hi-Tech Park, 250 Bi-Bo Road, Shanghai 201203, China;3. Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China;4. Division of Research and Development, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa 250-0862, Japan;5. Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa City, Kanagawa 252-0880, Japan;1. Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, Punjab, India;2. Chembiotech Laboratories, Advanced Science & Technology Institute, Kyrewood House, Tenbury Wells, Worcestershire, WR1 8SG, UK;1. State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China;2. The New Zealand Institute of Plant & Food Research Limited, Motueka 7198, New Zealand;1. Department of NanoBiotechnology, NanoGlycobiology Unit, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Vienna, Austria;2. Department of Pathophysiology and Allergy Research, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria;3. Department of Pediatrics, Respiratory and Allergic Disease Division, Medical University Graz, Auenbruggerplatz 34, 8036 Graz, Austria;4. Department of Dermatology, University Hospital St. Pölten, Karl Landsteiner University of Health Sciences, Propst-Führer-Strasse 4, 3100 St. Pölten, Austria;1. A.N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33/2, Moscow, 119071, Russian Federation;2. Komarov Botanical Institute of the Russian Academy of Sciences, Professor Popov St. 2, St. Petersburg, 197376, Russian Federation;3. N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin St. 3, Moscow, 117809, Russian Federation;1. School of Engineering and Materials Science, Queen Mary University of London, London, UK;2. School of Electrical and Electronic Engineering, Shiraz University of Technology, Shiraz, Iran;3. Department of Cardiology, London Chest Hospital, Barts and the London NHS Trust, London, UK;4. Parks College of Engineering, Aviation and Technology, Saint Louis University, St. Louis, MO, USA |
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| Abstract: | The acquisition of amino acids by Lactobacillus delbrueckii subsp. bulgaricus 2038 (Lb. bulgaricus 2038) when grown in the presence of bovine casein, the major protein in bovine milk, was investigated by examining the expression of genes related to proteolysis and amino acid biosynthesis. To support the growth on bovine casein, Lb. bulgaricus 2038 has to synthesise five kinds of amino acids de novo, as proteolysis from casein does not provide these. The incomplete hydrolysis in combination with amino acids biosynthesis may explain the slow growth of Lb. bulgaricus 2038 in a casein environment. Meanwhile, it was determined that Lb. bulgaricus 2038 uses different intracellular peptidases when grown in casein or whey medium, and initially yields the important amino acid glutamate from the C-terminal or N-terminal end of peptides imported into the cell. |
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