Synthesis,characterization and comparative study of nano-Ag–TiO2 against Gram-positive and Gram-negative bacteria under fluorescent light |
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| Affiliation: | 1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi''an 710069, People''s Republic of China;2. College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471022, People''s Republic of China;3. School of Material Science and Engineering, Beihang University, XueYuan Road No. 37, HaiDian District, Beijing 100191, People''s Republic of China;1. Department of Materials Engineering and Center for Thin Film Technologies and Applications, Ming Chi University of Technology, Taipei 243, Taiwan;2. Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan;3. Institute of Materials Science and Engineering, National Taiwan University, Taipei 106, Taiwan;4. Electronics and Opto-electronics Research Laboratories, Industrial Technology Research Institute, Hsinchu 310, Taiwan |
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| Abstract: | Currently, some of the major problems affecting the world are air pollution as well as microbial contamination. Every time we breathe, we are risking our lives by inhaling dangerous chemicals and biological contaminants that have found their way into the air. Therefore this work focuses on the antibacterial activity of Ag–TiO2 to overcome the microbial contaminant and infectious disease. Ag–TiO2 nanosolution were synthesized by sol–gel method and found to be an effective visible light driven photocatalyst. The nanosolutions were characterized by X-ray diffraction (XRD), transmittance electron microscopy (TEM), photoluminescence (PL) spectra, and X-ray photoelectron spectroscopy (XPS). At the concentration of 0.2–0.1 M, Ag–TiO2 caused 100% inhibition of bacterial growth. The antibacterial efficacy of Ag–TiO2 was evaluated with two kinds of bacteria; Gram-positive and Gram-negative. The colony count of Ag–TiO2 against Gram-negative were evaluated with stain such as Escherichia coli, Pseudomonas aeruginosa and Shigella while for Gram-positive were investigated with Staphylococcus aureus, Bacillus subtilis, Bacillus cereus. Colony count results indicated that Ag–TiO2 able to kill bacteria at the lowest concentration of 0.05 M that contains 0.06 mol % Ag. From the SEM and TEM observation, the survival of the Gram-positive was low and the decomposition was rapid as compared to Gram-negative bacteria. |
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| Keywords: | Gram-negative bacteria Gram-positive bacteria Cytoplasmic membrane Photocatalyst |
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