Affiliation: | 1. Department of Food Chemistry, Faculty of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, GR-15784 Athens, Greece;2. Department of Ecology and Systematics, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece;1. INRA UMR1324, Centre des Sciences du Gout et de l’Alimentation, F-21000 Dijon, France;2. Université de Bourgogne, Dijon, France;3. INSERM U965, Hôpital Lariboisière, F-75475 Paris, France;4. Université Paris 7, F-75475 Paris, France;5. Service d’Anatomopathologie, Hôpital Lariboisière, F-75475 Paris, France;1. Department of Food Engineering, King Mongkut''s University of Technology Thonburi, Bangkok 10140, Thailand;2. Department of Physics, Syracuse University, Syracuse, NY 13244-1130, USA;1. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, 210023, China;2. Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023, China;3. School of Agriculture and Food Science, Zhejiang Forestry University, Hangzhou, 311300, China |
Abstract: | Aflatoxin B1 (AFB1) mostly produced by Aspergillus flavus and Aspergillus parasiticus, is an extremely toxic and carcinogenic metabolite. Currants are used in the Mediterranean diet as a food with antioxidant properties. Four strains of Aspergillus section Nigri have been isolated from currants originated from Crete and Corinth. In this study AFB1 production by A. parasiticus and the four strains of Aspergillus section Nigri in Cretan and Corinthian currants (Vitis vinifera L.) is investigated. AFB1 determination was performed by HPLC–FID. Results revealed that the four strains Aspergillus section Nigri, as well as the aflatoxigenic strain A. parasiticus produced AFB1 (0.0052–1.31 μg AFB1 15 g−1, corresponding to 0.0003–0.087 μg AFB1 g−1) in both type of currants (Cretan and Corinthian) on the 12th day of observation. Moreover, AFB1 production, by A. parasiticus in the synthetic Yeast Extract Sucrose (YES) medium was also studied. The ability of AFB1 production has been affected by the special characteristics of each isolate and the currants substrate. |