Fluorescent detection of clenbuterol using fluorophore functionalized gold nanoparticles based on fluorescence resonance energy transfer |
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| Affiliation: | 1. College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, PR China;2. Department of Endocrinology, First Hospital of Jiaxing (Affiliated Hospital of Jiaxing University), 1882 Zhonghuan South Road, Jiaxing, Zhejiang, 314001, PR China;3. College of Medicine, Jiaxing University, Jiaxing, Zhejiang, 314001, PR China;4. Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur, Chennai, 603203, Tamil Nadu, India;5. Department of Material Engineering, KU Leuven, Kasteelpark Arenberg 44, P.O. Box 2450, B-3001, Heverlee, Belgium;6. Key Laboratory of Optical Field Manipulation of Zhejiang Province, Department of Physics, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China;7. State Key Laboratory of Pollution Control and Resource Reuse, International Joint Research Center for Sustainable Urban Water System, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, PR China;1. College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, China;2. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;1. College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China;2. Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xinning Road, Xining, 810008, Qinghai, China;1. Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China;2. Technology Center of Qinhuangdao Customs, Qinhuangdao 066004, China;3. Tropical Medicine Institute & South China Chinese Medicine Collaborative Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China |
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| Abstract: | A new fluorescence method for detecting clenbuterol based on gold nanoparticles (AuNPs) and rhodamine B (RB) has been developed. It has been observed that the quenching of fluorescence of RB occurs in the present of AuNPs through the fluorescence resonance energy transfer (FRET). In the presence of clenbuterol, the FRET-based fluorescence of RB and AuNPs would be gradually recovered for the reason that clenbuterol could congregate the AuNPs and inhibit the FRET process. An assay for the determination of clenbuterol has been proposed based on the modulation in FRET efficiency between RB and AuNPs. Under the optimum conditions, the fluorescence recovery efficiency of the assay is proportional to the concentration of clenbuterol. The detection limit in swine feed was calculated to be 0.008 mg/g and the recoveries were in the range of 97%–102%. In short, the proposed method provides advantages such as rapidity, simplicity, low cost and high sensitivity, making it promising for the rapid screening of clenbuterol residues. |
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| Keywords: | Clenbuterol Fluorescence resonance energy transfer Gold nanoparticles Rhodamine B Swine feed |
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