Casamino Acids and Oxyrase enhance growth of Listeria monocytogenes in multi-pathogen enrichments |
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| Affiliation: | 1. Microbial Biophysics and Residue Chemistry Research Unit, USDA-ARS, Eastern Regional Research Center, Wyndmoor, PA 19038, USA;2. Nutrition, Food Safety/Quality, USDA-ARS, George Washington Carver Center, Beltsville, MD 20705, USA;1. Research Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai 600034, Tamil Nadu, India;2. Department of Bacteriology, National Institute for Research in Tuberculosis, ICMR, Sathyamoorty Road, Chetpet, Chennai 31, Tamil Nadu, India;1. Department of Chemical Engineering, Indian Institute of Science, Bangalore, 560612, India;2. Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India;1. Department of Chemistry, State Key Laboratory of Synthetic Chemistry, The University of Hong Kong, Hong Kong Special Administrative Region;2. Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region;3. Faculty of Science, University of Alberta, Edmonton, Alberta, Canada;1. Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, 8010 Graz, Austria;2. Insitute of Hygiene and Microbiology, University of Wuerzburg, Josef-Schneider Str. 2 E1, Wuerzburg 97080, Germany;3. Department of Paediatrics, Oxford University Hospitals, Headington, Oxford OX3 9DU, United Kingdom;1. Department of Food Science, Islamic Azad University, Tehran-North Branch, Tehran, Iran;2. Department of Food Science and Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran, Karaj, Iran;3. Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks, Iran;4. Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;5. School of Packaging, Michigan State University, East Lansing, MI 48824, USA;6. Food Technology Area, Universidad de Salamanca, E.P.S de Zamora, Avda. Requejo 33, 49022, Zamora, Spain |
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| Abstract: | Rapid methods have been developed as relatively faster alternatives to plate culture for the detection of pathogenic bacteria in foods. However, since most rapid methods are subject to logistical limitations (e.g., sample volume size, analysis time, matrix effects) and/or a detection scheme with insufficient sensitivity needed to detect very low levels of bacteria in foods, culture enrichment is often employed to increase the concentration of targeted pathogens prior to detection. Multiplexed rapid detection platforms, capable of simultaneous detection of different bacteria in a single sample, necessitate co-enrichment (or mixed culture enrichment) of as many different targeted microorganisms as possible in a timely manner. This investigation compares the growth of four major foodborne pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, and Yersinia enterocolitica) inoculated into pristine media or ground pork and enriched in various culture media. Initial results revealed that, after 24 h incubation, the growth of L. monocytogenes (the slowest-growing pathogen examined) was increased by approximately 1-log by the supplementation of Universal Preenrichment Broth with Casamino Acids and/or Oxyrase. Overnight (24 h) growth of L. monocytogenes in ground pork enrichment cultures was enhanced up to ca. 2-log by the addition of either Casamino Acids or Casamino Acids and Oxyrase for each of the tested growth media. Ultimately, an overnight culture of the inoculated pathogens in any of the selected media containing both Casamino Acids and Oxyrase was observed to yield target bacterial concentrations that were at sufficient levels (between 10e5 and 10e6 CFU/mL) for detection by most rapid methods. |
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| Keywords: | Casamino Acids Mixed culture Oxyrase Pathogens Rapid methods |
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