多西紫杉醇对人喉癌细胞Hep-2增殖的抑制作用

目的探讨多西紫杉醇(Docetaxel,TXT)对人喉癌细胞Hep-2增殖的抑制作用。方法分别用不同浓度的TXT(0、10、20、40、80、100和200ng/ml)作用Hep-2细胞不同时间(0、0.5、1、2和4h),MTT法检测细胞的增殖活性,并与阿霉素(Adriamycin,ADM)、5-氟尿嘧啶(5-fluorouracil,5-Fu)和长春新碱(Vincristine,VCR)等抗肿瘤药物的抑制效果进行比较。采用流式细胞术检测TXT对Hep-2细胞凋亡的影响。结果与ADM、5-Fu和VCR等抗肿瘤药物相比,TXT对Hep-2细胞具有较高的抑制活性,其抑制效果在一定范围内呈量效和时效关系;TXT能够有效诱导Hep-2细胞凋亡。结论 TXT可显著抑制Hep-2细胞增殖,是一种具有广阔应用前景的喉癌治疗临床药物。

Inhibitory Effect of Docetaxel on Proliferation of Human Laryngeal Carcinoma Hep-2 Cells

Objective To investigatetheinhibitoryeffectofdocetaxel(TXT)ontheproliferationofhuman laryngealcarcinoma Hep-2 cells.Methods Hep-2 cells were treated with TXT at various concentrations for 0,0.5,1,2 and 4 h separately.The proliferative activity of Hep-2 cells was determined by MTT method and compared with those of cells treated with adriamycin(ADM),5fluorouracil(5-Fu)and vincristine(VCR).The effect of TXT on apoptosis of Hep-2 cells was determined by flow cytometry.Results Compared with ADM,5-Fu and VCR,TXT showed high inhibiting activity to Hep-2 cells in dosage-and time-dependent modes at certain ranges.Meanwhile,TXT induced the apoptosis of Hep-2 cells effectively.Conclusion TXT inhibited the proliferation of Hep-2 cells significantly,which might be used as a potential drug for clinical therapy of laryngeal carcinoma.