| 应用C30-HPLC-PDA分离与鉴定食品中全反式叶黄素和玉米黄素 |
| |
| 引用本文： | 惠伯棣,李京,裴凌鹏.应用C30-HPLC-PDA分离与鉴定食品中全反式叶黄素和玉米黄素[J].食品科学,2006,27(9):151-154. |
| |
| 作者姓名： | 惠伯棣 李京 裴凌鹏 |
| |
| 作者单位： | 北京联合大学应用文理学院； 首都师范大学生命科学学院； 中国中医研究院 |
| |
| 基金项目： | 国家食品安全重大科技专项;北京市教委科技发展计划项目 |
| |
| 摘 要： | 在装备了二极管阵列检测器(PDA)的高压液相色谱(HPLC)上,应用C30柱,食品中的全反式叶黄素和玉米黄素获得了良好的分离。分离的色谱条件为:WatersYMCCarotenoidS-5(4.6×250mm)柱;乙腈-甲醇-三乙胺(75:25:0.05)为流动相A,甲基叔丁基醚-三乙胺(100:0.05,)为流动相B,流动相B在20min内由0%线性增至55%;流速为1.0ml/min;PDA波长范围为260～700nm;进样量为20μl。根据其色谱行为和光谱特征,全反式叶黄素和玉米黄素可被定性。这一结果使二者在C30-HPLC-PDA上的定量成为可能。
|
| 关 键 词： | 叶黄素 玉米黄素 C30柱 |
| 文章编号： | 1002-6630（2006）09-0151-04 |
| 收稿时间： | 2005-11-23 |
| 修稿时间： | 2005-11-23 |
Separation and Identification of All E-lutein and E-zeaxanthin in Foods with C30-HPLC-PDA |
| |
| HUI Bo-di,LI Jing,PEI Ling-peng.Separation and Identification of All E-lutein and E-zeaxanthin in Foods with C30-HPLC-PDA[J].Food Science,2006,27(9):151-154. |
| |
| Authors: | HUI Bo-di LI Jing PEI Ling-peng |
| |
| Affiliation: | 1.College of Applied Arts and Science, Beijing Union University, Beijing 100083, China; 2.College of Life Science, Capital Normal University, Beijing 100037, China; 3.China Academy of Traditional Chinese Medicine, Beijing 100009, China |
| |
| Abstract: | By HPLC equipped with PDA, all E-lutein and E-zeaxanthin in foods were readily separated on a C30 column under following conditions: YMCTM Carotenoid S-5 column (4.6×250mm); mobile phase A= acetonitrile-methanol-spasmolytol(75: 25:0.05); mobile phase B=methyl tert-butyl ether-spasmolytol (100:0.05); linear gradient: B increased from 0 to 55% in 20 minutes at the flow rate of 1.0 ml/min; PDA wavelengths range: 260～700nm and sample injection volume: 20μl. According to their chromatographic behavior and spectral characteristics, two fractions, all E-lutein and E-zeaxanthin are all identified. These results form the base to quantify those two fractions with C30-HPLC-PDA. |
| |
| Keywords: | lutein zeaxathin C_(30) column |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
