| 酸果蔓中SOD酶的提取及活性测定 |
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| 引用本文： | 毛绍春,李竹英,李聪.酸果蔓中SOD酶的提取及活性测定[J].食品与机械,2007,23(1):62-63,68. |
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| 作者姓名： | 毛绍春 李竹英 李聪 |
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| 作者单位： | 1. 玉溪农业职业技术学院,云南,玉溪,653100
2. 云南大学应用化学系,云南,昆明,650091 |
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| 摘 要： | 利用正交法探讨了磷酸缓冲液用量、pH值、提取时间、丙酮用量对酸果蔓SOD酶提取率的影响，并用改良Marklund法测定了酸果蔓中SOD的活性，当在酸果蔓中加入1．5倍的磷酸缓冲液，pH7.8，提取2h，再用1倍体积的丙酮沉淀，酸果蔓中SOD酶粗品的提取率达0．95％；酸果蔓中SOD酶单位体积的活力为433.7U／mL。酸果蔓中SOD酶含量高且活性强。
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| 关 键 词： | 酸果蔓 提取 活性测定 |
| 修稿时间： | 2006-09-12 |
Extraction and activity determination of cranberry SOD |
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| MAO Shao-chun,LI Zhu-ying,LI Cong.Extraction and activity determination of cranberry SOD[J].Food and Machinery,2007,23(1):62-63,68. |
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| Authors: | MAO Shao-chun LI Zhu-ying LI Cong |
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| Affiliation: | 1. Yuxi Agricultural Institute, Yuxi, Yunnan 653100,China; 2. The Department of Chemistry, Yunan University, Kunming, Yunnan 650091 ,China |
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| Abstract: | Effect of extraction rate on Cranberry superoxide dismutase (SOD) were discussed using orthogonal design, in which four factors have considered,that is,amount of phosphoric acid buffer solution(PBS), PH, extraction time and amount of acetone. activity of Cranberry SOD was determined by Marklund method. Experimental results were as fdlows:Extraction rate of Cranberry SOD up to 0.95%, when add 1.5 time of phosphoric acid buffer solutions in Cranberry, pH7.8, extraction 2 h and 1 time of volumes the acetone precipitations. Cranberry SOD unit volume vigor is 433.7 U/mL. Cranberry has a very high SOD content with high activity. |
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| Keywords: | SOD |
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