| 蔬菜中赤霉素残留量的高效液相色谱检测方法研究 |
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| 引用本文： | 葛雅琨,张元新,胡睿,周艳明. 蔬菜中赤霉素残留量的高效液相色谱检测方法研究[J]. 吉林化工学院学报, 2005, 22(2): 13-16 |
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| 作者姓名： | 葛雅琨 张元新 胡睿 周艳明 |
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| 作者单位： | 吉林化工学院,化学工程系,吉林,吉林,132022;吉林化工学院,自动化工程系,吉林,吉林,132022;沈阳农业大学,分析测试中心,辽宁,沈阳,110161 |
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| 摘 要： | 使用高效液相色谱(HPLC)对蔬菜中的农药GA3残留进行分析检测.用80%甲醇溶液作为提取剂;建立了液液萃取与SPE PAKC18固相萃取相结合纯化GA3的方法;检测波长为206.00nm;流动相为甲醇与7.5mM甲酸水的体积比为3:7的溶液;实验结果表明,该方法是符合快速、可靠、灵敏、实用标准的GA3残留分析检测方法.
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| 关 键 词： | 高效液相色谱 赤霉素 残留检测方法 蔬菜 |
| 文章编号： | 1007-2853(2005)02-0013-04 |
| 修稿时间： | 2005-04-01 |
Research on analysis of remnant of GA3 in vegetables by HPLC |
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| GE Ya-kun,ZHANG Yuan-xin,HU Rui,ZHOU Yan-ming. Research on analysis of remnant of GA3 in vegetables by HPLC[J]. Journal of Jilin Institute of Chemical Technology, 2005, 22(2): 13-16 |
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| Authors: | GE Ya-kun ZHANG Yuan-xin HU Rui ZHOU Yan-ming |
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| Affiliation: | GE Ya-kun~1,ZHANG Yuan-xin~2,HU Rui~3,ZHOU Yan-ming~3 |
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| Abstract: | Artificial GA_3 is widely used in agriculture.Large amount usage and illogical employment would be harmful to human health.Developing an analysis technology of the remnants of GA_3 is very important.But there is no appropriate means to detect the remnant of GA_3 presently.In this thesis, GA_3 in vegetable is firstly detected by HPLC.GA_3 in vegetable is purified by both liquid phase extraction and solid phase extraction.The best UVD wavelength to detect GA_3 is 206.00nm.The composition of flowage phase is methyl alcohol:7.5mM formic acid=3:7.Many experiments have been done to detect the feasibility of this method.The results prove that this method is fast, credible, sensitive and accords with the request for detection GA_3 remnant in vegetable. |
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| Keywords: | HPLC gibberellin vegetable method for remnant detection |
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