碱性甘露聚糖酶发酵培养基的优化

选用魔芋粉作为碳源，豆饼粉和谷氨酸钠作为氮源物质，对发酵培养基进行优化，并进行模型验证和5L发酵罐生产实验。结果表明：最佳发酵培养基(g/L，5L发酵罐)为魔芋粉12、豆饼粉15、酵母粉5、NaCl 84.4、谷氨酸钠3.11、K2HPO4 1.5、MgSO4 0.3、Na2CO3 10，起始pH 9.5～10.0，碱性β-甘露聚糖酶活力最高可达到2610.1U/mL。

Optimization of Fermentation Medium for Alkaline Mannanase Production

The fermentation medium for producing alkaline mannose using konjac flour as the carbon source and soybean cake powder and sodium glutamate as the nitrogen source was optimized by Pluckett-Burman (PB) design, steepest ascent test and central composite design. Based on a central composite design for three independent variables including NaCl and sodium glutamate concentrations, a predictive mathematical model was built and validated. Meanwhile, scale-up production experiments were done in a 5-L fermentor. Our results indicated that the optimal fermentation medium (g/L) was composed of konjac flour 12, soybean cake powder 15, yeast powder 5, NaCl 84.4, sodium glutamate 3.11, K2HPO4 1.5, MgSO4 0.3, Na2CO3 10, and initial pH 9.5-10.0, yielding an alkaline mannose as high as 2610.1 U/mL.