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Rapid determination of double-bond positions in monoenoic fatty acids by periodate-permanganate oxidation |
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| Authors: | Donald T. Downing Richard S. Greene |
| Affiliation: | (1) Departments of Dermatology and Biochemistry, Boston University School of Medicine, Boston, Massachusetts;(2) Department of Dermatology, Evans Memorial Department of Clinical Research, University Hospital, Boston University Medical Center, Boston, Massachusetts |
| Abstract: | Tetramethylammonium hydroxide has been used in the extraction and pyrolysis methylation of the carboxylic acids produced by periodate-permanganate oxidation of monounsaturated fatty acid methyl esters. This modification of the von Rudloff procedure allows rapid determination of double-bond positions and analysis of mixtures of positional isomers of monoenoic fatty acids. |
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