| 静电纺丝制备壳聚糖/聚己内酯血管支架及表征 |
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| 引用本文： | 杨文静,付静,何磊,王婷,王大新,何农跃.静电纺丝制备壳聚糖/聚己内酯血管支架及表征[J].复合材料学报,2011,28(1):104-108. |
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| 作者姓名： | 杨文静 付静 何磊 王婷 王大新 何农跃 |
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| 作者单位： | 1.东南大学 生物科学与医学工程学院, 南京 210096; |
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| 基金项目： | 国家重点基础研究发展计划(2007CB936104); 国家863计划(2007AA021905) |
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| 摘 要： | 结合壳聚糖(CS)和聚己内酯(PCL)二者的优点, 以静电纺丝的方法制备了CS/PCL血管支架。采用SEM和电子万能试验机检测了该支架的结构和力学性能, 将内皮祖细胞(EPCs)与该支架膜复合培养, 评估了该血管支架维持细胞黏附、 繁殖和分化的能力。SEM结果显示: 通过静电纺丝可以得到多孔、 类似于天然细胞外基质的直径约400nm的纤维微结构; 当CS与PCL质量比为0.5时, 静电纺丝所制备的CS/PCL血管支架弹性最大形变达到31.64%, 应力-应变曲线显示其弹性变形能力较强; EPCs在CS/PCL血管支架黏附率可达95.1%, 荧光显微镜观察结果也显示了CS/PCL血管支架利于细胞黏附、 生长。
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| 关 键 词： | 壳聚糖 聚己内酯 血管支架 静电纺丝 内皮祖细胞 |
| 收稿时间： | 2010-01-28 |
| 修稿时间： | 2010-05-08 |
Preparation and characterization of chitosan/polycaprolactone vascular scaffolds by electrospinning |
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| YANG Wenjing,FU Jing,HE Lei,WANG Ting,WANG Daxin,HE Nongyue.Preparation and characterization of chitosan/polycaprolactone vascular scaffolds by electrospinning[J].Acta Materiae Compositae Sinica,2011,28(1):104-108. |
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| Authors: | YANG Wenjing FU Jing HE Lei WANG Ting WANG Daxin HE Nongyue |
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| Affiliation: | 1.School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;2.School of Public Health Southeast, Southeast University, Nanjing 210096, China;3.Clinical Medical Colleges, Yangzhou University, Yangzhou 225001, China |
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| Abstract: | The chitosan/polycaprolactone(CS/PCL) vascular scaffolds were prepared by electrospinning in order to combine the advantage of chitosan(CS) and polycaprolactone(PCL) into the vascular scaffolds.The obtained CS/PCL vascular scaffolds were characterized by SEM and electronic universal testing machine.The endothelial progenitor cells(EPCs) were implanted in the scaffolds with various mass ratios of CS to PCL.The vascular scaffolds were examined by adhesion rate in different culturing times and the cells breedi... |
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| Keywords: | chitosan polycaprolactone vascular scaffold electrospinning endothelial progenitor cells |
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