| PCR法制备地高辛标记探针斑点杂交检测鳗弧菌 |
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| 引用本文： | 秦蕾,徐静,张正. PCR法制备地高辛标记探针斑点杂交检测鳗弧菌[J]. 淮海工学院学报, 2008, 17(2) |
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| 作者姓名： | 秦蕾 徐静 张正 |
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| 作者单位： | 淮海工学院江苏省海洋生物技术重点建设实验室 江苏连云港222005(秦蕾，徐静)，中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室 山东青岛266071(张正) |
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| 基金项目： | 淮海工学院校科研和教改项目 |
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| 摘 要： | 以鳗弧菌(Vibrio anguillarum)toxR基因内366~571 bp之间的基因序列为靶序列,采用PCR法制备对鳗弧菌特异性的地高辛标记DNA探针,探针长度为206 bp。选取2株鳗弧菌和8株与鳗弧菌亲缘关系非常接近的常见水产动物致病弧菌,通过斑点杂交法对制备的探针进行特异性检测,结果显示该探针对鳗弧菌杂交阳性,而与弧菌属的其它8株细菌均无交叉反应。这表明该地高辛标记探针具有很强的特异性,它能够很好地对鳗弧菌感染进行检测。
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| 关 键 词： | 鳗弧菌 PCR法 地高辛标记探针 斑点杂交 |
Detection of Vibrio anguillarum by Dot Blot Hybridization with Digoxigenin Labeled Probe by PCR |
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| QIN Lei,XU Jing,ZHANG Zheng. Detection of Vibrio anguillarum by Dot Blot Hybridization with Digoxigenin Labeled Probe by PCR[J]. Journal of Huaihai Institute of Technology:Natural Sciences Edition, 2008, 17(2) |
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| Authors: | QIN Lei XU Jing ZHANG Zheng |
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| Abstract: | A digoxigenin labeled DNA probe was produced by PCR method using toxR gene between 366 bp and 571 bp of Vibrio anguillarum as a target.The length of probe was 206 bp.Together with two V.anguillarum strains,ten pathogenic vibrio strains were chosen to detect the specificity of probe for V.anguillarum by dot blot hybridization.Result showed that the probe was able to hybridize with V.anguillarum.And no hybridization signals were observed using other eight vibrio strains most close to V.anguillarum,which demonstrated that the probe had strong specificity and had high value in detecting V.anguillarum. |
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| Keywords: | V.anguillarum PCR digoxigenin labeled DNA probe dot blot hybridization |
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