| LED诱导人血液荧光光谱研究 |
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| 引用本文： | 彭长德,高淑梅,刘莹,兰秀风,沈中华,陆建,倪晓武.LED诱导人血液荧光光谱研究[J].应用激光,2003,23(4):232-234. |
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| 作者姓名： | 彭长德 高淑梅 刘莹 兰秀风 沈中华 陆建 倪晓武 |
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| 作者单位： | 1. 徐州师范大学物理系江苏,徐州,221009;南京理工大学理学院,南京,210094
2. 南京理工大学理学院,南京,210094 |
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| 摘 要： | 用 4 0 8nm的LED诱导不同浓度人全血溶液的荧光光谱 ,包括研究了血液的自吸收对其荧光光谱结构产生的影响 ;分析了血液的荧光光谱随其浓度增加出现红移的现象并进行了解释 ;提出了 5 5 6nm谱峰不是某一荧光团所产生的特征峰 ,而是由吸收所造成的假峰的观点 ;讨论了激励光散射中心波长偏离激励光中心波长现象的原因。研究结果对光诱导生物组织自体荧光诊断技术有一定参考价值
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| 关 键 词： | 吸收光谱 荧光光谱 全血 荧光团 红移 |
Investigation of LED-induced Fluorescent Spectra from Human Blood Solution |
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| Peng Changde , Gao Shumei , Liu Ying , Lan Xiufeng Lu Jian Ni Xaowu.Investigation of LED-induced Fluorescent Spectra from Human Blood Solution[J].Applied Laser,2003,23(4):232-234. |
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| Authors: | Peng Changde Gao Shumei Liu Ying Lan Xiufeng Lu Jian Ni Xaowu |
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| Affiliation: | Peng Changde 1,2 Gao Shumei 1,2 Liu Ying 1,2 Lan Xiufeng 2 Lu Jian 2 Ni Xaowu 2 |
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| Abstract: | The fluorescent spectra of human blood solution with different concentrations induced by 408nmLED are reported in this paper, including the investigation of the influence on fluorescent profile exerted by blood self-absorption. The phenomena that fluorescence spectral peaks are apparently red-shifted with the increment of blood concentration are analyzed and the mechanism is given a reasonable explanation. The viewpoint is put forward that 556nm spectrum is illusive owing to blood absorption but not a characteristic fluorescence emitted by a certain fluorophore. The reasons that the central wavelength of dispersion light departures the one of exciting-light are discussed. The research result may have reference value to light induced tissue autofluorescence diagnosis techniques. |
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| Keywords: | absorption spectra fluorescent spectra whole blood fluorophore red-shifted |
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