Conjugated Linoleic Acid Reduces Hepatic Steatosis and Restores Liver Triacylglycerol Secretion and the Fatty Acid Profile During Protein Repletion in Rats |
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Authors: | María F Andreoli Paola G Illesca Marcela A González Claudio A Bernal |
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Affiliation: | 1.Bromatología y Nutrición, Departamento de Ciencias Biológicas, Facultad de Bioquímica y Ciencias Biológicas,Universidad Nacional del Litoral,Santa Fe,Argentina;2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET),Santa Fe,Argentina |
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Abstract: | Protein depletion is associated with hepatic steatosis and decreased circulating triacylglycerol (TAG). Since conjugated linoleic
acid (CLA) increases lean body mass, protects against muscle catabolism, and modulates lipid metabolism, the aim of this work
was to investigate the effects of CLA with two different amounts of dietary fat on the regulation of plasma and hepatic TAG
concentration, and its possible connections with changes in fatty acid (FA) profile in plasma, liver and adipose tissue and
hepatic oxidative status during protein repletion. Rats were fed a low protein diet (14 days) and then a protein repletion
diet (30 days), supplemented or not with CLA, containing 7% (w/w) or 20% (w/w) of fat. Hepatic TAG secretion and removal by
muscle and adipose tissue lipoprotein lipase, FA profile and liver oxidative status were evaluated. Protein depletion affected
hepatic TAG secretion and peripheral removal, decreasing plasma and increasing liver TAG concentration, whereas protein repletion
with CLA improved these abnormalities independently of the amount of dietary fat by increasing hepatic TAG secretion. This
prevention in the absence of CLA was not observed. CLA was incorporated in plasma and tissues (adipose > liver > plasma, and
c9,t11-CLA > t10,c12-CLA), accompanied by alterations in FA composition, mainly in adipose tissue. The hepatic oxidative stress was overcome
by protein repletion. CLA had a beneficial impact on TAG metabolism in protein repleted animals, preventing hepatic steatosis
through higher hepatic TAG secretion. |
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