| 产谷胱甘肽酵母菌株培养基优化 |
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| 引用本文： | 陈俭梅,袁建国,刘代成. 产谷胱甘肽酵母菌株培养基优化[J]. 食品与药品, 2009, 11(5) |
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| 作者姓名： | 陈俭梅 袁建国 刘代成 |
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| 作者单位： | [1]山东师范大学,山东济南250014 [2]山东省食品发酵工业研究设计院,山东济南250013 |
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| 摘 要： | 目的筛选对谷胱甘肽产量影响显著的碳源和氮源，考察其浓度对产量的影响。方法通过单因素实验和正交试验确定最佳碳源、氮源和无机盐浓度。结果最佳培养基的基本组成：2．5％葡萄糖，1％酵母粉，0．3％硫酸铵，0．03％硫酸镁，1．2g／L磷酸氢二钾和1．8g／L磷酸二氢钾。结论确定了最优培养基组成。
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| 关 键 词： | 谷胱甘肽 酵母菌 培养基优化 |
Medium Optimization of Glutathione-producing Strain |
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| CHEN Jian-mei,YUAN Jian-guo,LIU Dai-cheng. Medium Optimization of Glutathione-producing Strain[J]. Food and Drug, 2009, 11(5) |
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| Authors: | CHEN Jian-mei YUAN Jian-guo LIU Dai-cheng |
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| Affiliation: | 1.Shandong Normal University;Jinan 250014;China;2.Shandong Food and Fermentation Industry Re-search and Design Institute;Jinan 250013;China |
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| Abstract: | Objective To conduct the screening of carbon source and nitrogen source and investigate their influences on the yield of glutathione.Methods The single factor and orthogonal experiments were adopted to obtain the optimal concentrations of carbon source,nitrogen source and inorganic salts.Results The optimal medium composition was as follows:2.5 % dextrose,1 % yeast powder,0.3 %(NH4)2SO4,0.03 % MgSO4,1.2 g/L K2HPO4 and1.8 g/L KH2PO4.Conclusion The optimal medium composition can be determined. |
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| Keywords: | glutathione yeast medium optimization |
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