近临界水中鱼肉水解制备氨基酸的反应动力学

利用鱼肉蛋白制备氨基酸不但具有经济效益,而且为生物质资源高效利用提供技术支持。采用HL-F(0.2 L+1.5 MG)/30 MPa-IIA超临界水反应装置,在无催化剂、反应温度分别为220,240,260℃,反应时间为30 min条件下,对鱼肉蛋白在近临界水中水解为氨基酸的反应动力学进行了实验研究。用AAA-Direct氨基酸分析仪测定不同反应时间中氨基酸总产率,以酸水解鱼肉蛋白得到的氨基酸量为完全水解标准。在水过量的情况下,得到了鱼肉蛋白水解率宏观反应动力学方程。结果表明鱼肉蛋白水解动力学的级数为1.614 7,220,240,260℃下的反应速率常数分别为0.001 7,0.004 5,0.009 7,活化能为145.125 kJ/mol,前置因子A为9.475 7×109,为工业化生产提供了基础数据。

Reaction kinetics of fish meat hydrolysis for amino acids production in sub-critical water

Amino acids production by using fish protein not only has economic benefits,but also provides technical support for efficient biomass use.The reaction kinetics of fish protein hydrolysis for amino acids production in sub-critical water reactor model HL-F(0.2 L+1.5 MG)/30 MPa-IIA without catalysts was studied with the reaction time of 30 min and the experimental temperatures of 220,240 and 260 ℃ respectively.The total yield of amino acids at any time in hydrolysis reaction was determined by AAA-Direct analyzer.The max amino acids yield came from fish protein entire hydrolysis by hydrochloric acid.According to these experimental data and under the condition of water excess,the macroscopic reaction kinetics equation of fish protein hydrolysis was obtained.The experimental results show that the hydrolysis reaction order is 1.6147,and the reaction velocity constants are 0.0017,0.0045 and 0.0097 at 220,240 and 260 ℃ respectively.The activation energy is 145.125 kJ/mol and the Arrhenius pre-exponential factor A is 9.4757×109.These results supply the basic data for industrial production.