| 一株产几丁质酶渤海丝状真菌（Alternariatenuissima）的筛选及鉴定 |
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| 引用本文： | 刘丹,孟凡欢,李爽,冯欣,孟庆恒,孙建华. 一株产几丁质酶渤海丝状真菌（Alternariatenuissima）的筛选及鉴定[J]. 中国酿造, 2012, 31(2): 45-48 |
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| 作者姓名： | 刘丹 孟凡欢 李爽 冯欣 孟庆恒 孙建华 |
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| 作者单位： | 天津师范大学 生命科学学院,天津市细胞遗传与分子调控重点实验室,天津300387 |
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| 基金项目： | 国家自然科学基金资助项目(30971904) |
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| 摘 要： | 以渤海湾水域获得的52株丝状海洋真菌为出发菌株，通过平板透明圈法和发酵液酶活性测定，从中筛选出一株产几丁质酶活力较高的海洋真菌mTJ04-1．1，结合形态学方法和18SrDNA．ITS序列分析对其进行鉴定，确定该菌为极细链格孢霉（AltemariatenuissimamTJ04-1-1）。该菌能在以几丁质为唯一碳源的平板上生长并产生明显透明圈，透明圈与菌落直径的比值（D／d）达N2．81。对发酵液几丁质酶活力分析结果表明，该酶最适反应温度为45℃，最适pH值为6．5，最适条件下酶活性可达3．2U／mL。
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| 关 键 词： | 渤海丝状真菌 几丁质酶 筛选 鉴定 18S rDNA．ITS序列 |
Screening and identification of chitinase-producing fungus(Alternaria tenuissima) from Bohai Bay |
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| LIU Dan , MENG Fanhuan , LI Shuang , FENG Xin , MENG Qingheng , SUN Jianhua. Screening and identification of chitinase-producing fungus(Alternaria tenuissima) from Bohai Bay[J]. China Brewing, 2012, 31(2): 45-48 |
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| Authors: | LIU Dan MENG Fanhuan LI Shuang FENG Xin MENG Qingheng SUN Jianhua |
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| Affiliation: | (College of Life Science,Tianjin Normal University,Tianjin Key Laboratory of Cyto-Genetical & Molecular Regulation; Tianjin 300387,China) |
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| Abstract: | A chitinase-producing strain of marine-derived fungus mTJ04-1-1 was isolated from 52 isolated strains from Bohai-Bay through transparent circle method and chitinase activity determination.The strain was identified as Alternaria tenuissima based on its morphological characteristics and 18S rDNA-ITS sequence.Further experiment proved that mTJ04-1-1 strain grew well on the chitin cultural plates with visible transparent circle.The ratio of transparent circle to colony(D/d) was 2.81.Activity of the chitinase reached 3.2 U/ml under optimal temperature 45℃ and pH value 6.5. |
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| Keywords: | Bohai filamentous fungi chitinase screening identification 18S rDNA-ITS sequence |
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