| 海藻酸钠固定化亚油酸异构酶及其酶学性质初步研究 |
| |
| 引用本文： | 游庆红,尹秀莲.海藻酸钠固定化亚油酸异构酶及其酶学性质初步研究[J].中国酿造,2012,31(3):45-47. |
| |
| 作者姓名： | 游庆红 尹秀莲 |
| |
| 作者单位： | 1. 淮阴工学院 生命科学与化学工程学院,江苏淮安223001;南京工业大学 食品与轻工学院,江苏南京210009
2. 淮阴工学院 生命科学与化学工程学院,江苏淮安,223001 |
| |
| 基金项目： | 淮安市科技支撑项目(SN1160) |
| |
| 摘 要： | 以海藻酸钠为载体固定化亚油酸异构酶。研究了海藻酸钠浓度、酶用量、CaC12浓度、固定化时间对固定化过程的影响。结果表明,最佳固定化工艺是：以4%的海藻酸钠为载体、应用海藻酸钠溶液用量与酶液量的体积比3：1、3%的CaC12固定化5h。固定化酶的最适pH值为7.0,与游离酶相比,提高了0.5个pH单位;固定化酶和游离酶最适温度分别为35℃和30℃;固定化酶比游离酶具有更好的温度和pH值适应性。
|
| 关 键 词： | 海藻酸钠 亚油酸异构酶 固定化酶 |
Optimization of conditions for immobilizing linoleic acid isomerase by sodium alginate and its enzymatic characteristics |
| |
| YOU Qinghong
,
YIN Xiulian.Optimization of conditions for immobilizing linoleic acid isomerase by sodium alginate and its enzymatic characteristics[J].China Brewing,2012,31(3):45-47. |
| |
| Authors: | YOU Qinghong YIN Xiulian |
| |
| Affiliation: | 1(1.College of Life Sciences and Chemical Engineering;Huaiyin Institute of Technology;Huaian 223001,China; 2.College of food science and light industry;Nanjing university of technology;Nanjing 210009,China) |
| |
| Abstract: | Linoleic acid isomerase was immobilized using sodium alginate as the carrier.The immobilized conditions were studied.The optimal immobilization conditions were as followed: sodium alginate 4%,the volume ratio of sodium alginate to enzyme dosage 3:1,CaC12 3% and immobilized time 5h.Under these conditions,the optimum pH of the immobilized enzyme was shifted from 6.5 to 7.0,and the optimum reaction temperature of immobilized and free enzyme were 35℃ and 30℃,respectively.The immobilized enzyme had a high adaptability to temperature and pH value. |
| |
| Keywords: | sodium alginate linoleic acid isomerase immobilized enzyme |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
