产碱性脂肪酶菌株的筛选和培养

以Rodamine B平板筛选法获得了一株高活力碱性脂肪酶产生菌Pseudomonas sp. ZJU-02.摇瓶产酶实验表明，适宜产酶培养基为(%)：玉米浆3，植物油1, K2HPO4 0.1, KCl 0.05, MgSO4 0.05, Tween80 0.05；最佳培养条件为温度26℃、初始pH 6.5. 脂肪酶活力可高达86 IU/ml. 该酶在70℃以下、pH 7.0~10.5范围内稳定；酶反应的适宜温度为40℃，适宜pH为9.5. 该酶制剂在洗涤剂和制革工业中有良好的应用前景.

Screening and Culture of Alkaline Lipase-produced Strain Pseudomonas sp. ZJU-02

A strain Pseudomonas sp. ZJU-02 was screened from 7 strains. The strain had high lipase producing ability proved by the Rodamine B plate method. The results in shake flask cultivation showed that the suitable lipase producing media were: corn steep 3%, vegetable oil 1%, K2HPO4 0.1%, KCl 0.05%, MgSO4 0.05%, Tween 80 0.05%. Under optimal culture conditions (26oC and pH 6.5), the maximal lipase activity could reach 86 IU/ml. This enzyme was stable under 70oC and pH 7~10.5. The optimal conditions for the enzymatic reaction were 40oC and pH 9.5. This enzyme showed a good potential application in detergent and tan industry.