HPLC法测定盐酸格拉司琼鼻喷剂的含量

目的：建立盐酸格拉司琼鼻喷剂中盐酸格拉司琼的含量的高效液相色谱测定法。方法：采用Phenomenex prodigy 5μ ODS（3）（250×4．6mm）色谱柱，以醋酸钠缓冲液（0．05mol·L^-1，含0．25％三乙胺．冰醋酸调PH至6．0）-甲醇（50：50）为流动相，流速：1mL·min^-1，检测波长为301nm。结果：盐酸格拉司琼线性范围为0．2-20μg（r=0．99996）．平均回收率为100．75％，RSD=1.01％（n=3）。结论：本方法具有操作简单，分析快捷准确，干扰少等优点。

Determination of Content of Granisetron Hydrochloride Nasal Spray by HPLC

Objective ：A HPLC method for the determination of content of Granisetron Hydrochloride Nasal Spray was established.METHODS A Phenomenex prodigy 5μ ODS （3） column was used with sodium acetate buffer 0.05mo1·l^-1,with 0.25% Triethlamine,pH adjusted to 6.0 by acetic acid]and methanol （50：50）as mobile phase.The flow rate was 1 ml·min^-1 and detection wavelength 301nm.Resuhs ：The linear range of Granisetron Hydroehloride was 0.2-20μg,r=0.99996.The average recovery rate was 100.75% ,The corresponding RSD=1.01%. Conclusion：The method was simplicity, accuracy and little interference.