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| 香菇子实体多糖分离纯化的研究 | |
| 引用本文： | 燕航,钟耀广,王淑琴,刘长江. 香菇子实体多糖分离纯化的研究[J]. 食品研究与开发, 2006, 27(8): 61-63 |
| 作者姓名： | 燕航 钟耀广 王淑琴 刘长江 |
| 作者单位： | 1. 大连轻工业学院生物与食品工程学院,辽宁,大连,116034 2. 大连轻工业学院生物与食品工程学院,辽宁,大连,116034;沈阳农业大学食品学院,辽宁,沈阳,110161 3. 沈阳农业大学食品学院,辽宁,沈阳,110161 |
| 摘 要： | 将香菇子实体干粉经热水浸提、乙醇沉淀,得到香菇多糖粗品。香菇多糖粗品经Sevage法去除游离蛋白,H2O2脱色,用DEAE-纤维素柱层析,经蒸馏水、0.05mol/LNaCl、0.1mol/LNaCl、0.2mol/LNaCl、0.3mol/LNaCl和0.4mol/LNaCl洗脱,得到6个香菇多糖级分A～F;然后用SephadexG-200柱进一步纯化香菇多糖B级分,得香菇多糖亚级分B-1和B-2;再研究其中的B-1。采用SephdexG-200柱层析和聚丙烯酰胺凝胶电泳法,证实该多糖组分为均一多糖。 |
| 关 键 词： | 香菇多糖 分离 纯化 |
| 收稿时间： | 2006-04-27 |
| 修稿时间： | 2006-04-27 |
STUDIES ON TECHNOLOGY OF PURIFICATION OF LENTINAN |
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| YAN Hang,ZHONG Yao-guang,WANG Shu-qin,LIU Chang-jiang. STUDIES ON TECHNOLOGY OF PURIFICATION OF LENTINAN[J]. Food Research and Developent, 2006, 27(8): 61-63 | |
| Authors: | YAN Hang ZHONG Yao-guang WANG Shu-qin LIU Chang-jiang |
| Affiliation: | 1. College of Biology and Food Technology, Dalian Institute of Light Industry, Dalian 116034, Liaoning, China; 2. Department of Food Technology,Shenyang Agricultural University, Shenyang 110161, Liaoning, China |
| Abstract: | Crude lentinan was obtained from fruitbody of lentinus edodes using following methods: extracting polysaccharide with hot water, precipitating with ethyl alcohol. Free proteins and pigment were removed by Sevage method and H2O2. Six lentinan fractions were got by DEAE-Cellulose column with eluent of water, 0.05 mol/L, 0.1 mol/L, 0.2 mol/L, 0.3 mol/L and 0.4 mol/L NaCl respectively. Letinan-B was further purified on Sephadex G-200 column, two subfractions of letinan (B-1, B-2)were obtained. And letinan B-1 would be studied, too.Sephadex G-200 column chromatography and polyacrylamide gel electrophoresis illustrated that the fraction B-1 was homogenous polysaccharide. |
| Keywords: | lentinan isolated purify |
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