| 正相干柱和反相柱色谱组合快速分离纯化长春瑞滨技术研究 |
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| 引用本文： | 赵春芳,吴泽强,余龙江,李硕,熊茵.正相干柱和反相柱色谱组合快速分离纯化长春瑞滨技术研究[J].现代化工,2007,27(5):42-44. |
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| 作者姓名： | 赵春芳 吴泽强 余龙江 李硕 熊茵 |
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| 作者单位： | 华中科技大学生命科学与技术学院,资源生物学与生物技术研究所,武汉430074 |
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| 摘 要： | 采用正相干柱硅胶色谱与反相聚苯乙烯(MCI-GELCHP-20)柱色谱相结合的方法,将化学半合成抗癌药物长春瑞滨粗产物进行快速分离纯化。结果显示绝大部分粗原料中长春碱衍生物、反应中间产物、以及产物伴生杂质可快速与长春瑞滨分离,产物纯度达98%以上。该法具有快速、节省溶剂、分离效果好、回收率高等优点。
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| 关 键 词： | 长春瑞滨 正相干柱色谱 反相色谱 分离纯化 |
| 文章编号： | 0253-4320(2007)05-0042-03 |
| 修稿时间： | 2007年1月20日 |
Study on separation and purification of vinorelbine in tandem using normal dry-column flash and reversed-phase chromatography |
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| ZHAO Chun-fang,WU Ze-qiang,YU Long-jiang,LI Shuo,XIONG Yin.Study on separation and purification of vinorelbine in tandem using normal dry-column flash and reversed-phase chromatography[J].Modern Chemical Industry,2007,27(5):42-44. |
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| Authors: | ZHAO Chun-fang WU Ze-qiang YU Long-jiang LI Shuo XIONG Yin |
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| Abstract: | The normal dry-column flash and MCI-GEL CHP-20 reversed-phase chromatography was used to quickly separate and purify vinorelbine,an anticancer drug semi-synthesized by the chemical reaction.Results indicated that most ramification of vinca alkaloid in source material,intermediates created during the process of chemosynthesis and accompanying impurities can be separated from vinorelbine quickly,the purity of end product can be up tp 98%.This method of separation has high efficiency with quick speed,low solvent cost,and high recovery. |
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| Keywords: | vinorelbine reversed-phase chromatography dry-column flash chromatography separation and purification |
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