Enzymatic Conditions of an In Vitro Method to Study Protein Digestion |
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| Authors: | S F GAUTHIER C VACHON L SAVOIE |
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| Affiliation: | Authors Vachon and Savoie are with the Département de nutrition humaine, Centre de recherche en nutrition, Facultédes sciences de l'agriculture et de l'alimentation, UniversitéLaval, Quebec Canada G1K 7P4. Author Gauthier is with the Centre de recherche en nutrition and the Centre de recherches alimentaires de Saint-Hyacinthe, Direction de la recherche, Agriculture Canada, Saint-Hyacinthe, Québec, Canada J2S 4Z4. Direct inquires to Dr. Savoie. |
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| Abstract: | An in vitro digestion method was proposed for studying protein digestion. Protein (casein) was submitted to peptic proteolysis in a closed system followed by hydrolysis with pancreatic enzymes in a “digestion cell” with continuous elimination of digested products by dialysis. The peptic digestion was performed with a pepsin source of high specific activity (3152 units/mg protein) with an enzyme:substrate (E:S) ratio of 1: 250. The second-step of proteolysis was carried out with pancreatin for 6 hr at an E:S ratio of 1:25. A 10 mM sodium phosphate buffer, pH 7.5, was used as the circulating dialysis buffer. |
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