结合基因工程的生物电子鼻用于气味检测的研究

为了实现结合生物工程的仿生嗅觉传感器系统的多气味检测和目标气味的特异性识别和区分，使用生物工程化的大鼠嗅觉系统作为敏感元件，植入嗅球的多通道微丝电极作为换能器，采用无线数据采集系统收集和分析响应信号。最后，利用Matlab计算神经元平均放电率，对神经元的气味响应进行相关性分析和主成分分析，证实了该系统检测和区分不同气味的性能。创新性地提出将嗅觉受体基因整合、克隆到在体嗅觉细胞上，用于改造生物嗅觉系统，使之可以对目标气味分子产生敏感的响应信号。结果表明结合基因工程的生物电子鼻系统能够显著增强其检测特异性目标气味的性能，在环境监测、公共安全等领域中有很大应用潜力。

Development of a Bioelectronic Nose with Genetic Engineering for the Odor Detection

In order to realize the multi-odor detection and the specific identification and differentiation of target odors using the bioengineered bionic olfactory sensor system,a bioengineered rat olfactory system is used as a sensitive element,a multi-channel micro wire electrodes implanted in the olfactory bulb is used as a transducer,and a wireless data acquisition system is used to collect and analyze the responsive signals.Finally,Matlab is used to calculate the mean firing rate of neurons,and the correlation analysis and principal component analysis of the odor responses of neurons are performed,which confirmed the performance of the system in detecting and distinguishing different odors.It is innovatively proposed to integrate and clone the olfactory receptor genes into in-vivo olfactory cells to transform the biological olfactory system so that it can generate sensitive responsive signals to target odor molecules.The results show that the biological electronic nose system combined with genetic engineering is able to significantly enhance its performance in detecting specific target odors,and has great potential for applications in many fields such as environmental monitoring,public safety.