珍贵橙色束丝放线菌固体发酵合成安丝菌素P-3的工艺探索

为了探索珍贵橙色束丝放线菌（Actinosynnema pretiosum）合成安丝菌素P-3（AP-3）的固体发酵培养条件的最佳工艺，利用农副产品（玉米淀粉、玉米粉、棉籽饼粉、豆粕、豆饼粉、菜籽饼粉、麦麸、米糠）作为固料，采用单因素实验和正交实验对固体发酵培养基组成及培养条件进行摸索。得到合成AP-3固体发酵的最佳培养基组分为：玉米淀粉、豆粕及棉籽饼粉的质量配比为1：1：1，红糖4%，玉米浆干粉2%，CaCl₂ 1%。最佳固体发酵工艺条件为：初始pH为7.5，培养温度28℃，固液比（质量比）为1：0.8，装量25g（250mL锥形瓶），接种量150mL/kg，培养时间为7天，最优条件下AP-3的产量为（26.86±1.87）mg/kg（每千克培养基）。本研究为发酵合成安丝菌素提供了新的方法与思路。

Optimal process of solid-state fermentation for ansamitocin P-3 production by Actinosynnema pretiosum

Solid-state fermentation of Actinosynnema pretiosum was investigated to establish an optimal process for synthesis of AP-3. The agricultural and sideline products (cornstarch, cornmeal, cottonseed meal, soybean meal, bean cake powder, rapeseed meal, wheat bran and rice bran) were used as raw materials. The compositions of solid fermentation medium and culture condition optimization were studied through single factor experiment and orthogonal design. The optimal mediums for the solid-state fermentation of AP-3 production was as follows (w/w):cornstarch, soymeal and cottonseed mass ratio 1:1:1, 4% of brown sugar, 2% of corn steep powder, 1% of CaCl₂. The optimal conditions for solid fermentation were as follows:initial pH 7.5, culture temperature 28℃, solid-liquid ratio (mass ratio) 1:0.8, quality of solid medium 25g (250mL conical flask), inoculum volume 150mL/kg, incubation time 7d. Under the optimal conditions, the yield of AP-3 could reach (26.86±1.87)mg/kg (per kilogram of medium). This study provided a new method and idea for the solid-state fermentation for ansamitocin P-3 production.