Characterization of the cultivable microbial community in a spinach-processing plant using MALDI-TOF MS |
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Authors: | Lena Hausdorf Kerstin Mundt Michaela Winzer Christiana Cordes Antje Fröhling Oliver Schlüter Michael Klocke |
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Affiliation: | 1. Leibniz-Institut für Agrartechnik Potsdam-Bornim e.V., Max-Eyth-Allee 100, 14469 Potsdam, Germany;2. Hochschule Anhalt, Bernburger Straße 55, 06366 Köthen, Germany |
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Abstract: | A better and regular control of the production chain of fresh fruits and vegetables is necessary, because a contamination of the product by human- and phyto-pathogenic microorganisms may result in high losses during storage and poses a threat to human health. Therefore, detailed knowledge about the occurrence and the diversity of microorganisms within single processing steps is required to allow target-oriented produce safety control. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was successfully used to identify bacterial colonies. Bacteria can be identified with high accuracy by comparing them with generated spectra of a reference database. |
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Keywords: | Post-harvest processing Vegetables Bacteria Identification Whole cell MALDI-TOF mass spectra 16S rRNA gene nucleotide sequence analysis |
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