高效液相法同时测定土茯苓中五种多酚成分

本文建立了土茯苓中五种多酚成分：5-O-咖啡酰莽草酸、落新妇苷、花旗松素、黄杞苷及白藜芦醇的高效液相色谱分析方法。色谱柱为Agilem Zorbax SB Cl8柱（250mm×4．6mmi．d．，5μm），流动相为乙腈（A）及O．1％醋酸水溶液（B），线性梯度洗脱程序为0～15mm，16～21％A；15～40min，21～40％A。五种成分线性良好（r〉998），保留时间相对标准偏差介于0122～0．76%峰面积相对标准偏差介于2．67～4．64%，加标回收率介于96．5～103．7％。对18批土茯苓样品分析结果表明，落新妇苷是土茯苓中最主要黄酮成分，含量范围为5．48～5．75mg/g；花旗松素、黄杞苷、异黄杞苷、落新妇苷及其三种异构体（以落新妇苷计）等七种黄酮总量介于6．72～35．3mg/g，平均含量为18士8．5mg/g；5-O-咖啡酰莽草酸含量也较高，含量范围为0．9～6．29mg/g，该成分在土茯苓中的含量测定为首次报道。但花旗松素和白藜芦醇含量较低，在一些样品中未检测到。

Quantitative Analysis of Five Polyphenols in Rhizoma smilacis Giabrae by HPLC

A HPLC quantitative method for simultaneous determination of 5-O-caffeoylshikimic acid, astilbin, taxifolin, engeletin and trans-resveratrol in Rhizoma Smilacis Glabrae （RSG） was developed. An Agilent Zorbax SB C18 column （250 mm×4.6 mm i.d., 5 pm） was used. The mobile phase consisted of acetonitrile （A） and 0.1% acetic acid aqueous solution 03） with linear gradient program of 16～21% （A） in 0～15 rnin, 21～40% （A） in 15～40 min. The calibration curve of each analyte was constructed under six concentrations. The correlation coefficients were all 〉0.998, and the RSD values of ta and the peak area ranged from 0.22 to 0.76% and from 2.67 to 4.64%, respectively. Among 18 RSG samples, astilbin was the most dominant constituent with content ranged from 5.48 to 25.75 mg/g. The total amount of seven flavonols including taxifolin, engeletin, isoengeletin, astilbin and its three isomers （quantified as astilbin） was in the range of 6.72-35.3 mg/g, with average of 18＋8.5 mg/g. 5-O-caffeoylshikimic acid was the second dominant component with content ranged from 0.97 to 6.29 mg/g, while the contents of taxifolin and trans-resveratrol were relatively low, and in some samples was even undetected.