核桃粕、核桃仁酶解物抗氧化活性的研究

比较核桃粕、核桃粕蛋白提取物、核桃仁、去皮核桃仁、脱脂核桃仁和脱脂去皮核桃仁酶解物的抗氧化活性。结果表明，各样品酶解物还原力大小顺序为：核桃仁〉核桃粕蛋白提取物〉核桃粕﹥脱脂核桃仁〉去皮核桃仁〉脱脂去皮核桃仁。当底物浓度为10 mg/mL时，核桃仁直接酶解物的还原力达到谷胱甘肽的79.74%。ORAC值大小顺序为：核桃仁〉脱脂核桃仁〉核桃粕蛋白提取物〉核桃粕〉去皮核桃仁〉脱脂去皮核桃仁。核桃仁直接酶解物的ORAC值最大，为1560.75 μmol Trolox equivalent/g Peptide，与等质量的谷胱甘肽的ORAC值相当。各样品对H2O2诱导PC12细胞损伤均有保护效果，其中核桃仁直接酶解物的保护效果最好，当底物浓度为0.10 mg/mL、0.25 mg/mL和0.50 mg/mL时，均比其它样品的细胞存活率高，分别为72.64%、90.43%和84.98%；当底物浓度为1.00 mg/mL时，脱脂核桃仁酶解物的保护效果最好，细胞存活率为85.11%。

The Antioxidant Activities of Walnut Protein and its Enzymolysis Product

The antioxidant activities of walnut dreg, walnut dreg protein extract, walnut kernel, peeled walnut kernel, defatted walnut kernel, peeled and defatted walnut kernel hydrolysates were researched. The results showed that, the order of reducing power of each hydrolysate was as follows： walnut kernel ？ walnut dreg protein extract ？ walnut dreg ？ defatted walnut kernel ？ peeled walnut kernel ？ peeled and defatted walnut kernel. When the substrate concentration was 10 mg/mL, the reducing power of walnut kernel hydrolysate was 79.74% of the glutathione. The sequence of ORAC value was as followed： walnut kernel ？ defatted walnut kernel ？ walnut dreg protein extract ？ walnut dreg ？ peeled walnut kernel ？ peeled and defatted walnut kernel. Walnut kernel hydrolysate had the highest ORAC value 1560.75 μmol Trolox equivalent/g peptide, which was equal to the same weight of the glutathione. All the enzymolysis products could protect PC12 cell damage induced by H2O2, compared with other hydrolysates, the walnut kernel had the best protective effect. When its substrate concentration was 0.10 mg/mL,0.25 mg/mL and 0.50 mg/mL, the cell survival rate reached 72.64%, 90.43% and 84.98% respectively. When the substrate concentration was 1.00 mg/mL, the best protective effect was defatted walnut kernel with the cell survival rate being of 85.11%.