灵芝深层发酵中麦角甾醇含量的测定

利用HPLC法研究了灵芝真菌发酵过程中胞内外麦角固醇的含量，经紫外全波长扫描，确定测定波长为282mm，研究表明：此方法测定麦角固醇含量最大标准偏差为O．92％，得到线性回归方程为y=28685x-382．61（R=0．9998），该回归方程在20～500嵋的范围内。精密度试验，灵芝真菌发酵胞内外麦角固醇含量RsD分别为O．40％和1．96％，说明此试验设计条件精密度良好，经加标后，麦角固醇的回收率在96．82～102．50％之间，表明此方法用于测定灵芝真菌发酵过程中麦角固醇含量准确、可行，经测定灵芝真菌发酵至120h，麦角固醇含量增至最高，胞内含量达到O．64mg／g，胞外含量达到0．07mg／mL。

Determination of Ergosterol by HPLC in the Fermentation of Ganoderma lucidum

The ergosterol content in fermentation ofGanoderma lucidum was determined by HPLC at 282 nm. The results showed that the maximum standard deviation was 0.92% and the linear regression equation y= 28685x-382.61 （R=0.9998） in the range of 20～500 pg. The precision test showed that Ganoderma lucidum extraceUular and intracellular ergosterol content of RSD were 0.40% and 1.96%, respectively. The spike recoveries for ergosterol were between 96.82 and 102.50%. The method was feasibility and accurate for determination of ergosterol content in fermentation of Ganoderma lucidum. The ergosterol content of Ganoderma lucidum extracellular and intracellular were 0.07 mg/mL and 0.64mg/g, respectively, after fermentation for 120 h.