大肠癌细胞辐射敏感相关蛋白的初步筛选

人大肠癌细胞LOVO和SW480细胞具有不同的辐射敏感性。用直线加速器分别予以0、2、4、6GyX射线照射，然后运用SELDI-TOF蛋白质芯片技术测定照射后24h细胞的蛋白质谱，分析两株细胞间在不同照射剂量后的蛋白质表达情况，通过Swiss-Prot数据库搜索初步筛选出可能与大肠癌细胞辐射敏感性相关的几种蛋白：GADD45B、Thioredoxin、Metallothionein1(MT1)。结果表明：大肠癌辐射敏感性的预测能够从蛋白质水平来体现，而SELDI-TOF蛋白质芯片技术可从蛋白质水平预测人大肠癌细胞辐射敏感性，为临床大肠癌患者的个体化放疗提供选择依据。

Preliminary Screening of Radiosensitivity-Associated Proteins in Colorectal Cancer Cells

Two human colorectal cancer cell lines with different degrees of radiosensitivity, LOVO and SW480, were received a succession of 0, 2, 4, and 6 Gy X-ray radiation. Then, in 24 h after radiation, the protein spectra of each cell line were measured by SELDI-TOF protein chip so that we could get the differentiated protein expressions of each cell line after different radiation doses. And the differentially-expressed protein peaks showing gradient change in a dose-dependent manner of the two cell lines were detected. Finally, GADD45B, Thioredoxin and Metallothionein 1 (MT1) were screened out through Swiss-Prot database as proteins highly related with radiosensitivity in colorectal cancer. The result shows that radiosensitivity in colorectal cancer can be predicted at protein level and SELDI-TOF protein chip can be used to predict radiosensitivity in colorectal cancer at protein level, thus providing ample choices for individualized radiotherapy.