Towards an Ideal In Cell Hybridization-Based Strategy to Discover Protein Interactomes of Selected RNA Molecules |
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| Authors: | Michele Spiniello Mark Scalf Amelia Casamassimi Ciro Abbondanza Lloyd M. Smith |
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| Affiliation: | 1.Department of Precision Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy;2.Division of Immuno-Hematology and Transfusion Medicine, Cardarelli Hospital, 80131 Naples, Italy;3.Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; (M.S.); (L.M.S.) |
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| Abstract: | RNA-binding proteins are crucial to the function of coding and non-coding RNAs. The disruption of RNA–protein interactions is involved in many different pathological states. Several computational and experimental strategies have been developed to identify protein binders of selected RNA molecules. Amongst these, ‘in cell’ hybridization methods represent the gold standard in the field because they are designed to reveal the proteins bound to specific RNAs in a cellular context. Here, we compare the technical features of different ‘in cell’ hybridization approaches with a focus on their advantages, limitations, and current and potential future applications. |
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| Keywords: | RNA molecules, ‘ in cell’ hybridization methods, RNA– protein interactions |
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