Genome-Wide Classification and Phylogenetic Analyses of the GDSL-Type Esterase/Lipase (GELP) Family in Flowering Plants |
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Authors: | Alberto Cenci,Mairenys Concepció n-Herná ndez,Valentin Guignon,Geert Angenon,Mathieu Rouard |
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Affiliation: | 1.Bioversity International, Parc Scientifique Agropolis II, 34397 Montpellier, France;2.Instituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas (UCLV), Carretera a Camajuaní km 5.5, Santa Clara C.P. 54830, Villa Clara, Cuba;3.Research Group Plant Genetics, Vrije Universiteit Brussel (VUB), Pleinlaan 2, 1050 Brussels, Belgium |
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Abstract: | GDSL-type esterase/lipase (GELP) enzymes have key functions in plants, such as developmental processes, anther and pollen development, and responses to biotic and abiotic stresses. Genes that encode GELP belong to a complex and large gene family, ranging from tens to more than hundreds of members per plant species. To facilitate functional transfer between them, we conducted a genome-wide classification of GELP in 46 plant species. First, we applied an iterative phylogenetic method using a selected set of representative angiosperm genomes (three monocots and five dicots) and identified 10 main clusters, subdivided into 44 orthogroups (OGs). An expert curation for gene structures, orthogroup composition, and functional annotation was made based on a literature review. Then, using the HMM profiles as seeds, we expanded the classification to 46 plant species. Our results revealed the variable evolutionary dynamics between OGs in which some expanded, mostly through tandem duplications, while others were maintained as single copies. Among these, dicot-specific clusters and specific amplifications in monocots and wheat were characterized. This approach, by combining manual curation and automatic identification, was effective in characterizing a large gene family, allowing the establishment of a classification framework for gene function transfer and a better understanding of the evolutionary history of GELP. |
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Keywords: | gene families GDSL-type esterase/lipase plant genomes orthology database bioinformatics data curation male sterility pest and diseases |
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