柱前衍生化高效液相色谱-荧光检测法测定水产品中氨苄青霉素和阿莫西林残留

建立了柱前衍生化高效液相色谱-荧光检测法同时测定水产品中氨苄青霉素和阿莫西林含量。经固相萃取柱净化的样品通过衍生后,采用高效液相色谱荧光法测定。确定氨苄青霉素和阿莫西林线性范围为0.001~0.1 mg/kg（r=0.999 9）,方法检出限分别为0.001 mg/kg和0.005 mg/kg（S/N=3）,定量限分别为0.003 mg/kg和0.015 mg/kg（S/N=10）,回收率为76.3%~96.8%,相对标准偏差（n=6）在0.63%~6.71%之间。该方法简便快速、结果准确,可作为测定水产品中氨苄青霉素和阿莫西林含量的有效方法。

Determination of Ampicillin and Amoxicillin Residues in Aquatic Product Using Reversed-phase High Performance Liquid Chromatography-fluorescence Detection with Pre-column Derivatization

A methd is developed for determination of ampicillin and amoxicillin residues in aquatic product using reversed-phase high performance liquid chromatography-fluorescence detection with pre-column derivatization.The sample extracts are cleaned up by solid-phase extraction cartridge and then derivatized with derivative agent.The results show that the calibration graphs is linear in the concentration rang of 0.001~0.1 mg/kg（r＝0.999 9）. The detection limit and quantification limit are 0.001 mg/kg（S/N=3） and 0.003 mg/kg（S/N=10） for ampicillin, 0.005 mg/kg（S/N=3） and 0.015 mg/kg（S/N=10） for amoxicillin.The average recoveries are between 76.3%~96.8% with relative standard deviations（RSDs） of 0.63%~6.71%（n=6）. The method is simple,rapid,sensitive and reproducible,it can be used for the routine analysis of the ampicillin and amoxicillin residues in aquatic product.