Visual Observation of Hydrolyzed Potato Starch Granules by α‐Amylase with Confocal Laser Scanning Microscopy |
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Authors: | Soottitantawat Apinan,Ikuta Yujiro,Yoshii Hidefumi,Furuta Takeshi,Pä ivi Myllä rinen,Pirkko Forssell,Kaisa Poutanen |
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Abstract: | Porous starch was produced by digestion of freeze‐dried potato starch with α‐amylase from Bacillus sp. The surface structure of the granules became perforated and in the interior of the granules a capsule‐like cavity was formed, i.e. the hydrolyzed starch can be used as an encapsulant. The structure change of the granules was observed with confocal laser scanning microscopy and scanning electron microscopy. The degree of starch hydrolysis could be correlated with the Avrami equation. The activation energy of starch hydrolysis by α‐amylase was 83 kJ/mol. |
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Keywords: | α ‐Amylase Starch granule Cavity CLSM SEM |
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