| 铜绿微囊藻Microcystis aeruginosa PCC7820生物钟基因的克隆及其蛋白相互作用分析 |
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| 引用本文： | 徐虹,郑锦乾,吴韩志.铜绿微囊藻Microcystis aeruginosa PCC7820生物钟基因的克隆及其蛋白相互作用分析[J].高技术通讯,2009,19(3). |
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| 作者姓名： | 徐虹 郑锦乾 吴韩志 |
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| 作者单位： | 厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,厦门,361005 |
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| 基金项目： | 国家自然科学基金，福建省高等学校新世纪优秀人才支持计划 |
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| 摘 要： | 为了深入研究铜绿微囊藻生物钟调控的分子机制,根据蓝藻钟基因的同源序列设计合成了简并引物,通过PCR和染色体步行的方法获得了铜绿微藻Microcystis aeruginosa PCC7820的生物钟基因簇kaiABC.kaiA基因全长873bp,编码一个由290αα组成,分子量大小为33.3kDa的蛋白;kaiB基因全长315bp,编码分子量大小为11.8kDa的蛋白;kaiC基因全长1563 bp,编码蛋白分子量大小为58.3kDa.Kai蛋白间的相互作用是昼夜节律计时产生的重要过程,利用酵母双杂交系统检测了三种Kai蛋白间的相互作用,结果表明KaiA、KaiB和KaiC蛋白自身及KaiA与KaiC、KaiB与KaiC之间都能发生明显的相互作用,而KaiB和KaiA之间没有检测到相互作用.
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| 关 键 词： | 铜绿微囊藻 生物钟基因 染色体步移 酵母双杂交 |
Studies on cloning of circadian clock genes and physical interaction among circadian clock proteins in Microcystis aeruginosa PCC7820 |
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| Xu Hong,Zhen Jinqian,Wu Hanzhi.Studies on cloning of circadian clock genes and physical interaction among circadian clock proteins in Microcystis aeruginosa PCC7820[J].High Technology Letters,2009,19(3). |
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| Authors: | Xu Hong Zhen Jinqian Wu Hanzhi |
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| Affiliation: | Xu Hong,Zhen Jinqian,Wu Hanzhi (The Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Engineering,School of Life Sciences,Xiamen University,Xiamen 361005) |
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| Abstract: | To study the circadian clock genes' regulation and their function in cyanobacteria, the circadian clock gene cluster kaiABC was cloned from Microcystis aeruginosa PCC7820 by chromosome walking. The kaiA gene posesses 873 base pairs and encodes a 33.3-kD protein with 290 amino acid. The kaiB (315bp) and kaiC genes (1563 bp) encode 11.8-kDa and 58.3-kD proteins, respectively. To study the function of Kai proteins and find their interacting proteins, the clock genes kaiA, kaiB and kaiC were subcloned into the ... |
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| Keywords: | Microcystis aeruginosa PCC7820 clock gene chromosome walking yeast two hybridization |
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