儿茶素对肾病综合征大鼠系膜细胞增生的影响

研究儿茶素对肾病综合征大鼠肾小球系膜细胞增生的影响,阐明儿茶素对肾病综合征大鼠疗效的可能机制。方法:20只SD(Sprague-Dawley)雌性大鼠随机分成对照组、肾病组、儿茶素预防组、儿茶素治疗组共四组。实验末应用生物化学法测定血清中白蛋白(Albumin, Alb)、总蛋白(Total protein, TP)、三酰甘油(Trilyceride, TG)、血尿素氮(Blood Urea Nitrogen, BUN)、及24h尿蛋白排泄量(24hrs urinary protein,24hrsUP),应用免疫组化检测肾系膜中细胞周期调控蛋白P21、P27、PCNA、CyclinD1及TGF-β1的表达水平。结果各组大鼠肾小球系膜细胞CyclinD1、PCNA、P21、TGF-β1表达水平较对照组明显增高(p均<0.01),P27水平明显降低(p<0.01)。与肾病组相比,儿茶素预防组和治疗组系膜细胞P21、PCNA、CyclinD1、TGF-β1表达水平明显降低(p均<0.01),而P27表达水平增高明显(p<0.01)。儿茶素预防组和儿茶素治疗组无明显差异。肾病组大鼠尿蛋白排泄量较对照组显著增加(p<0.01)。从血清白蛋白浓度来看,儿茶素预防组、儿茶素治疗组明显高于肾病组,而血清TG则明显降低(p均<0.01),BUN也略低于肾病组,以儿茶素预防组较明显。儿茶素预防组和儿茶素治疗组血清总蛋白水平较肾病组有显著增加(p<0.05)。结论:儿茶素通过抑制P21、PCNA、Cycli

Study on Effect of Catechin on Glomemlar Mesangial Cells Protiferation in Rats Nephrotic Syndrome

To study the effect of catechin on glomerular mesangial cells proliferation in rats nephrotic syndrome to elucidatethe related influence on nephrotic syndrome.Methods:20 female SD rats were randomly divided into four groups:control,nephrotic, catechin-prevented and catechin-treated.In the end of experiment, Alb. TP,TG,BUN in serum and 24hr urinaryprotein were detected by biochemical assay. The expressions of P21,P27,PCNA,CyclinD1,TGF-β1 related cell cycle regulatoryprotein in renal mesangial cells were measured by means of immunohistochemical technique. Results:compared with controlgroup,the related cell cycle regulatory proteins as P21,PCNA,CyclinD1,TGF-β1 expressions in glomerular mesangial cells werehigher in the other groups (all p<0.01),while P27 expression was lower(p<0.01).Controlled with nephrotic group, theexpressions of P21,PCNA,CyclinD1,TGF-β1 in catechin-prevented and catechin-treated group were significantly lower(all p<0.01),but P27 expression was obviously higher (p<0.01).There was no significant difference between catechin-prevented andcatechin-treated group. Urinary protein extraction by rats in nephritic group increased more than control group(p<0.01).As tothe concentration of Alb in serum, the catechin-prevented and catechin-treated groups were higher than nephrotic group,Theconcentration of TG also significantly decreased (p<0.01).BUN was a little lower than nephrotic group,especially in catechin-prevented group.The concentrations of TP in catechin-prevented and catechin-treated group higher than nephrotic group (p<0.05).Conclusion:catechin could inhibit the proliferation of glomerular mesangial cells, maintain glomerular permeability,de-crease the extraction of urinary protein,improve normal lipid metabolism,recover normal renal function and slow chronicprogressive kidney lesions through improving P27 expression and decreasing P21、PCNA、Cyclind1、TGF-β1 inglomerular messangial cells.