Host-dependent activation of IS1203v excision in Shiga toxin-producing Escherichia coli |
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Authors: | Kusumoto Masahiro Suzuki Rieko Nishiya Yoshiaki Okitsu Tadayuki Oka Masanori |
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Affiliation: | Tsuruga Institute of Biotechnology, Toyobo Co. Ltd., 10-24 Toyo-cho, Tsuruga, Fukui 914-0047, Japan. masahiro_kusumoto@bio.toyobo.co.jp |
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Abstract: | IS1203v is an insertion sequence (IS) which is identical to the most abundant IS elements in the genome of Escherichia coli O157:H7. However, there is no sequence homologous to IS1203v in the genome of E. coli K-12. We constructed a system to analyze the excision frequency of IS1203v, and demonstrated that the frequency in E. coli O157:H7 was approximately 10(5) times higher than that in E. coli K-12. We also investigated the excision frequencies of IS1203v in various E. coli isolates, and showed that the excision frequencies of IS1203v-possessing strains were approximately 10(3) times higher than those of IS1203v-nonpossessing strains. The results suggest that the IS1203v-possessing strains use a common system to enhance IS1203v excision. |
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Keywords: | Shiga toxin-producing Escherichia coli insertion sequence IS1203v |
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